Experiment description of studies that identified Stat1 in exosomes

1 Experiment ID 263 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63|CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 25242146     Organism Mus musculus Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino Journal name Molecular Cell Publication year 2014 Sample Neural stem cells Sample name NPCs - Th1 treated Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.20 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 264 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63|CD9 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 25242146     Organism Mus musculus Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino Journal name Molecular Cell Publication year 2014 Sample Neural stem cells Sample name NPCs - Th2 treated Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.20 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Stat1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SMURF2   Affinity Capture-Luminescence Homo sapiens 2 SMAD4   Affinity Capture-Luminescence Homo sapiens 3 ACVR1 90 Affinity Capture-Luminescence Homo sapiens 4 TGFBR1   Affinity Capture-Luminescence Homo sapiens 5 SMURF1 57154 Affinity Capture-Luminescence Homo sapiens View the network

Pathways in which Stat1 is involved

No pathways found