Gene description for Stat3
Gene name signal transducer and activator of transcription 3
Gene symbol Stat3
Other names/aliases 1110034C02Rik
AW109958
Aprf
Species Mus musculus
 Database cross references - Stat3
ExoCarta ExoCarta_20848
Vesiclepedia VP_20848
Entrez Gene 20848
UniProt P42227  
 Stat3 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stat3
Molecular Function
    sequence-specific DNA binding GO:0043565 ISO
    glucocorticoid receptor binding GO:0035259 ISO
    protein binding GO:0005515 IPI
    RNA polymerase II core promoter proximal region sequence-specific DNA binding GO:0000978 ISO
    transcription factor binding GO:0008134 ISO
    ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity GO:0004879 ISO
    RNA polymerase II repressing transcription factor binding GO:0001103 ISO
    transcription regulatory region DNA binding GO:0044212 ISO
    sequence-specific DNA binding RNA polymerase II transcription factor activity GO:0000981 IDA
    protein dimerization activity GO:0046983 IPI
    protein phosphatase binding GO:0019903 ISO
    DNA binding GO:0003677 ISO
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    protein kinase binding GO:0019901 IPI
    signal transducer activity GO:0004871 IEA
    CCR5 chemokine receptor binding GO:0031730 ISO
    RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001228 ISO
Biological Process
    transcription, DNA-templated GO:0006351 IEA
    stem cell maintenance GO:0019827 IMP
    response to estradiol GO:0032355 ISO
    regulation of transcription, DNA-templated GO:0006355 NAS
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of cell proliferation GO:0008285 IGI
    temperature homeostasis GO:0001659 IMP
    protein import into nucleus GO:0006606 ISO
    sexual reproduction GO:0019953 IMP
    growth hormone receptor signaling pathway GO:0060396 ISO
    response to cytokine GO:0034097 ISO
    positive regulation of growth factor dependent skeletal muscle satellite cell proliferation GO:1902728 ISO
    phosphorylation GO:0016310 IDA
    signal transduction GO:0007165 IEA
    regulation of mitochondrial membrane permeability GO:0046902 ISO
    acute-phase response GO:0006953 IEA
    positive regulation of ATP biosynthetic process GO:2001171 ISO
    interleukin-6-mediated signaling pathway GO:0070102 ISO
    negative regulation of cell death GO:0060548 ISO
    negative regulation of glycolytic process GO:0045820 IMP
    negative regulation of neuron migration GO:2001223 IGI
    astrocyte differentiation GO:0048708 IMP
    eye photoreceptor cell differentiation GO:0001754 IMP
    eating behavior GO:0042755 IMP
    cellular response to hormone stimulus GO:0032870 ISO
    radial glial cell differentiation GO:0060019 IMP
    positive regulation of transcription, DNA-templated GO:0045893 ISO
    positive regulation of Notch signaling pathway GO:0045747 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 ISO
    JAK-STAT cascade GO:0007259 TAS
    cytokine-mediated signaling pathway GO:0019221 NAS
    regulation of multicellular organism growth GO:0040014 IMP
    transcription from RNA polymerase II promoter GO:0006366 ISO
    intracellular receptor signaling pathway GO:0030522 ISO
    cell proliferation GO:0008283 IDA
    glucose homeostasis GO:0042593 IMP
    negative regulation of neuron death GO:1901215 ISO
    negative regulation of hydrogen peroxide biosynthetic process GO:0010730 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    nuclear chromatin GO:0000790 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 ISO
    intracellular GO:0005622 TAS
    nucleus GO:0005634 ISO
    plasma membrane GO:0005886 IDA
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Stat3 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stat3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EP300 2033
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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