Gene description for Psmc3
Gene name proteasome (prosome, macropain) 26S subunit, ATPase 3
Gene symbol Psmc3
Other names/aliases TBP-1
Species Mus musculus
 Database cross references - Psmc3
ExoCarta ExoCarta_19182
Entrez Gene 19182
UniProt O88685  
 Psmc3 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Psmc3
Molecular Function
    ATP binding GO:0005524 IEA
    proteasome-activating ATPase activity GO:0036402 IBA
    nucleotide binding GO:0000166 IEA
    TBP-class protein binding GO:0017025 IBA
    hydrolase activity GO:0016787 IEA
Biological Process
    protein catabolic process GO:0030163 IEA
    positive regulation of RNA polymerase II transcriptional preinitiation complex assembly GO:0045899 IBA
    blastocyst development GO:0001824 IMP
    ER-associated ubiquitin-dependent protein catabolic process GO:0030433 IBA
    positive regulation of proteasomal protein catabolic process GO:1901800 IBA
Subcellular Localization
    membrane GO:0016020 ISO
    nucleus GO:0005634 ISO
    proteasome complex GO:0000502 ISO
    cytosolic proteasome complex GO:0031597 IBA
    cytoplasm GO:0005737 ISO
    nuclear proteasome complex GO:0031595 IBA
    proteasome regulatory particle, base subcomplex GO:0008540 IBA
    proteasome accessory complex GO:0022624 IDA
    cytoplasmic mRNA processing body GO:0000932 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Psmc3 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Psmc3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Psmc3 is involved
PathwayEvidenceSource
Activation of NF-kappaB in B cells IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC/C:Cdc20 mediated degradation of Securin IEA Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 IEA Reactome
Asymmetric localization of PCP proteins IEA Reactome
AUF1 (hnRNP D0) destabilizes mRNA IEA Reactome
Autodegradation of Cdh1 by Cdh1:APC/C IEA Reactome
Autodegradation of the E3 ubiquitin ligase COP1 IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
CDT1 association with the CDC6:ORC:origin complex IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cross-presentation of soluble exogenous antigens (endosomes) IEA Reactome
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
degradation of AXIN IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
degradation of DVL IEA Reactome
Degradation of GLI1 by the proteasome IEA Reactome
Degradation of GLI2 by the proteasome IEA Reactome
ER-Phagosome pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
Hedgehog 'on' state IEA Reactome
Hedgehog ligand biogenesis IEA Reactome
Orc1 removal from chromatin IEA Reactome
Orc1 removal from chromatin IEA Reactome
Regulation of activated PAK-2p34 by proteasome mediated degradation IEA Reactome
Regulation of ornithine decarboxylase (ODC) IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
SCF-beta-TrCP mediated degradation of Emi1 IEA Reactome
Separation of Sister Chromatids IEA Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A IEA Reactome
Ubiquitin-dependent degradation of Cyclin D1 IEA Reactome





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