Gene description for Usp14
Gene name ubiquitin specific peptidase 14
Gene symbol Usp14
Other names/aliases 2610005K12Rik
Species Mus musculus
 Database cross references - Usp14
ExoCarta ExoCarta_59025
Entrez Gene 59025
UniProt Q9JMA1  
 Usp14 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Usp14
Molecular Function
    hydrolase activity GO:0016787 IEA
    peptidase activity GO:0008233 IEA
    endopeptidase inhibitor activity GO:0004866 ISO
    ubiquitin-specific protease activity GO:0004843 ISO
    ubiquitinyl hydrolase activity GO:0036459 IEA
    cysteine-type endopeptidase activity GO:0004197 IBA
    proteasome binding GO:0070628 ISO
    cysteine-type peptidase activity GO:0008234 IEA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of endopeptidase activity GO:0010951 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    protein deubiquitination GO:0016579 ISO
    negative regulation of ER-associated ubiquitin-dependent protein catabolic process GO:1903070 ISO
    synaptic transmission GO:0007268 IMP
    regulation of proteasomal protein catabolic process GO:0061136 ISO
    regulation of chemotaxis GO:0050920 ISO
    proteolysis GO:0006508 IEA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 IEA
    cytoplasmic membrane-bounded vesicle GO:0016023 ISO
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 ISO
    synapse GO:0045202 IDA
    proteasome complex GO:0000502 IEA
    cell surface GO:0009986 ISO
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified Usp14 in exosomes
Experiment ID 15
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
Isolation/purification methods Filtration
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
 Protein-protein interactions for Usp14
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Usp14 is involved
No pathways found

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