Gene description for Psme1
Gene name proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Gene symbol Psme1
Other names/aliases AW413925
PA28a
Species Mus musculus
 Database cross references - Psme1
ExoCarta ExoCarta_19186
Vesiclepedia VP_19186
Entrez Gene 19186
UniProt P97371  
 Psme1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Psme1
Molecular Function
    protein binding GO:0005515 IPI
    endopeptidase activator activity GO:0061133 IDA
Biological Process
    antigen processing and presentation of exogenous antigen GO:0019884 IMP
    regulation of proteasomal protein catabolic process GO:0061136 IBA
    regulation of G1/S transition of mitotic cell cycle GO:2000045 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IBA
    cytoplasm GO:0005737 IBA
    proteasome activator complex GO:0008537 IEA
 Experiment description of studies that identified Psme1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Psme1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Psmb7 19177
Co-fractionation Mus musculus
2 Psmc6 67089
Co-fractionation Mus musculus
3 Psme2 19188
Co-fractionation Mus musculus
Co-fractionation Mus musculus
4 Psmd2 21762
Co-fractionation Mus musculus
5 Psmd8 57296
Co-fractionation Mus musculus
6 Psma2 19166
Co-fractionation Mus musculus
7 Psmc2 19181
Co-fractionation Mus musculus
8 Psma3 19167
Co-fractionation Mus musculus
9 Psmd13 23997
Co-fractionation Mus musculus
10 Uchl5 56207
Co-fractionation Mus musculus
11 Psmb1 19170
Co-fractionation Mus musculus
12 Atxn1  
Affinity Capture-MS Mus musculus
13 Txnl1 53382
Co-fractionation Mus musculus
14 Ufd1l  
Co-fractionation Mus musculus
15 Vcp 269523
Co-fractionation Mus musculus
16 Psme3 19192
Co-fractionation Mus musculus
17 Psmd14 59029
Co-fractionation Mus musculus
18 Psmb8 16913
Co-fractionation Mus musculus
19 Psmc5 19184
Co-fractionation Mus musculus
20 Emd  
Co-fractionation Mus musculus
21 Psmc1 19179
Co-fractionation Mus musculus
22 Psmd5 66998
Co-fractionation Mus musculus
23 Usp14 59025
Co-fractionation Mus musculus
24 Eif6 16418
Co-fractionation Mus musculus
25 Psmd7 17463
Co-fractionation Mus musculus
26 Zfand2b  
Co-fractionation Mus musculus
27 Runx2  
Affinity Capture-MS Mus musculus
28 Psmc4 23996
Co-fractionation Mus musculus
29 Psmd3 22123
Co-fractionation Mus musculus
30 Rnf181  
Co-fractionation Mus musculus
31 Psmb10  
Co-fractionation Mus musculus
32 Psmd6 66413
Co-fractionation Mus musculus
33 Ubqln4  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Psme1 is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
Post-translational protein modification IEA Reactome
Proteasome assembly IEA Reactome





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