Gene description for Psme3
Gene name proteaseome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki)
Gene symbol Psme3
Other names/aliases AA410043
AU020960
Ki
PA28gamma
REGgamma
pa28g
Species Mus musculus
 Database cross references - Psme3
ExoCarta ExoCarta_19192
Vesiclepedia VP_19192
Entrez Gene 19192
UniProt P61290  
 Psme3 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Psme3
Molecular Function
    p53 binding GO:0002039 ISO
    p53 binding GO:0002039 ISS
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    endopeptidase activator activity GO:0061133 IBA
    endopeptidase activator activity GO:0061133 IEA
    endopeptidase activator activity GO:0061133 ISO
    MDM2/MDM4 family protein binding GO:0097371 ISO
    MDM2/MDM4 family protein binding GO:0097371 ISS
Biological Process
    apoptotic process GO:0006915 IEA
    regulation of proteasomal protein catabolic process GO:0061136 IBA
    regulation of proteasomal protein catabolic process GO:0061136 IEA
    regulation of proteasomal protein catabolic process GO:0061136 ISO
    regulation of G1/S transition of mitotic cell cycle GO:2000045 IBA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 ISO
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    proteasome activator complex GO:0008537 IEA
 Experiment description of studies that identified Psme3 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Psme3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Psma3 19167
Co-fractionation Mus musculus
2 Mafa  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 Psme1 19186
Co-fractionation Mus musculus
4 Psme2 19188
Co-fractionation Mus musculus
5 Tulp3  
Co-fractionation Mus musculus
6 Psmb1 19170
Co-fractionation Mus musculus
7 Atxn1  
Affinity Capture-MS Mus musculus
8 Lmo2  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
9 Foxp3  
Affinity Capture-MS Mus musculus
10 Ube2z  
Co-fractionation Mus musculus
11 Atg16l1  
Affinity Capture-MS Mus musculus
12 Runx2  
Affinity Capture-MS Mus musculus
13 Psmd14 59029
Co-fractionation Mus musculus
14 Psma2 19166
Co-fractionation Mus musculus
15 Klf2  
Affinity Capture-Western Mus musculus
16 Txn2  
Co-fractionation Mus musculus
17 Fancd2  
Affinity Capture-MS Mus musculus
18 Casp7  
Two-hybrid Mus musculus
Biochemical Activity Mus musculus
19 Casp3 12367
Biochemical Activity Mus musculus
20 Tcf3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Psme3 is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
Post-translational protein modification IEA Reactome
Proteasome assembly IEA Reactome





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