Gene description for Psmb8
Gene name proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7)
Gene symbol Psmb8
Other names/aliases Lmp-7
Lmp7
Species Mus musculus
 Database cross references - Psmb8
ExoCarta ExoCarta_16913
Vesiclepedia VP_16913
Entrez Gene 16913
UniProt P28063  
 Psmb8 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Psmb8
Molecular Function
    endopeptidase activity GO:0004175 IBA
    threonine-type endopeptidase activity GO:0004298 IEA
    protein binding GO:0005515 IPI
Biological Process
    antigen processing and presentation GO:0019882 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    fat cell differentiation GO:0045444 IMP
    fat cell differentiation GO:0045444 ISO
    proteolysis involved in protein catabolic process GO:0051603 IEA
    regulation of endopeptidase activity GO:0052548 ISO
Subcellular Localization
    proteasome complex GO:0000502 ISO
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    proteasome core complex GO:0005839 IDA
    proteasome core complex, beta-subunit complex GO:0019774 IBA
    proteasome core complex, beta-subunit complex GO:0019774 ISS
    spermatoproteasome complex GO:1990111 IDA
 Experiment description of studies that identified Psmb8 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Psmb8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Psmd9  
Co-fractionation Mus musculus
2 Psmd13 23997
Co-fractionation Mus musculus
3 Psmd8 57296
Co-fractionation Mus musculus
4 Psmd5 66998
Co-fractionation Mus musculus
5 Psme2 19188
Co-fractionation Mus musculus
6 Psme4  
Co-fractionation Mus musculus
7 Adrm1  
Co-fractionation Mus musculus
8 Psmd7 17463
Co-fractionation Mus musculus
9 Psmd2 21762
Co-fractionation Mus musculus
10 Psmc1 19179
Co-fractionation Mus musculus
11 Vcp 269523
Co-fractionation Mus musculus
12 Psmc4 23996
Co-fractionation Mus musculus
13 Psme1 19186
Co-fractionation Mus musculus
14 Psmd12 66997
Co-fractionation Mus musculus
15 Psmd3 22123
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Psmb8 is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
Post-translational protein modification IEA Reactome
Proteasome assembly IEA Reactome





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