Gene description for Nploc4
Gene name nuclear protein localization 4 homolog (S. cerevisiae)
Gene symbol Nploc4
Other names/aliases Npl4
Species Rattus norvegicus
 Database cross references - Nploc4
ExoCarta ExoCarta_140639
Entrez Gene 140639
UniProt Q9ES54  
 Nploc4 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Nploc4
Molecular Function
    protein complex binding GO:0032403 IPI
    zinc ion binding GO:0008270 IEA
    K63-linked polyubiquitin binding GO:0070530 IDA
    ubiquitin binding GO:0043130 IDA
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IDA
    K48-linked polyubiquitin binding GO:0036435 IDA
Biological Process
    membrane fusion GO:0061025 IDA
    retrograde protein transport, ER to cytosol GO:0030970 ISO
    ubiquitin-dependent protein catabolic process GO:0006511 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    Golgi organization GO:0007030 IDA
Subcellular Localization
    cytosol GO:0005829 IDA
    protein complex GO:0043234 ISO
    nucleus GO:0005634 ISO
    VCP-NPL4-UFD1 AAA ATPase complex GO:0034098 NAS
 Experiment description of studies that identified Nploc4 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nploc4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nploc4 is involved
No pathways found





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