Gene description for PFN1
Gene name profilin 1
Gene symbol PFN1
Other names/aliases -
Species Bos taurus
 Database cross references - PFN1
ExoCarta ExoCarta_513895
Entrez Gene 513895
UniProt P02584  
 PFN1 identified in exosomes derived from the following tissue/cell type
Milk 23459212    
Serum 19327352    
 Gene ontology annotations for PFN1
Molecular Function
    actin binding GO:0003779 IEA
Biological Process
    actin cytoskeleton organization GO:0030036 IEA
    regulation of transcription from RNA polymerase II promoter GO:0006357 ISS
    positive regulation of epithelial cell migration GO:0010634 IEA
    neural tube closure GO:0001843 ISS
    regulation of actin polymerization or depolymerization GO:0008064 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISS
    cytoskeleton GO:0005856 IEA
    nucleus GO:0005634 ISS
 Experiment description of studies that identified PFN1 in exosomes
1
Experiment ID 213
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23459212    
Organism Bos taurus
Experiment description Bovine milk proteome: Quantitative changes in normal milk exosomes, milk fat globule membranes and whey proteomes resulting from Staphylococcus aureus mastitis.
Authors Reinhardt TA, Sacco RE, Nonnecke BJ, Lippolis JD.
Journal name J Proteomics
Publication year 2013
Sample Milk
Sample name Staphylococcus aureus-infected-Milk
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Filtration
Sucrose density gradient
FACS
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 187
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19327352    
Organism Bos taurus
Experiment description Anchorage-independent growth of breast carcinoma cells is mediated by serum exosomes.
Authors Ochieng J, Pratap S, Khatua AK, Sakwe AM.
Journal name Exp Cell Res
Publication year 2009
Sample Serum
Sample name Normal - Fetal bovine serum
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PFN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTB 60
Co-crystal Structure Homo sapiens
View the network image/svg+xml
 Pathways in which PFN1 is involved
PathwayEvidenceSource
PCP/CE pathway IEA Reactome
Platelet degranulation IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Signaling by Robo receptor IEA Reactome





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