Gene description for PACS1
Gene name phosphofurin acidic cluster sorting protein 1
Gene symbol PACS1
Other names/aliases MRD17
Species Homo sapiens
 Database cross references - PACS1
ExoCarta ExoCarta_55690
Vesiclepedia VP_55690
Entrez Gene 55690
HGNC 30032
MIM 607492
UniProt Q6VY07  
 PACS1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PACS1
Molecular Function
    protein binding GO:0005515 IPI
    transmembrane transporter binding GO:0044325 IBA
    transmembrane transporter binding GO:0044325 IPI
Biological Process
    lymphocyte homeostasis GO:0002260 ISS
    protein localization to Golgi apparatus GO:0034067 IEA
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IMP
Subcellular Localization
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 TAS
    COPI-coated vesicle GO:0030137 IEA
 Experiment description of studies that identified PACS1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PACS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 PTPRG 5793
Affinity Capture-MS Homo sapiens
3 GGA3 23163
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 PPP1CB 5500
Affinity Capture-MS Homo sapiens
5 SORL1 6653
Affinity Capture-Western Homo sapiens
6 SNX24  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 FURIN 5045
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
8 TP53 7157
Affinity Capture-MS Homo sapiens
9 PPP1CA 5499
Affinity Capture-MS Homo sapiens
10 PDGFRA 5156
Affinity Capture-MS Homo sapiens
11 ATG4A  
Affinity Capture-MS Homo sapiens
12 VAMP4 8674
Affinity Capture-Western Homo sapiens
13 CSNK2A1 1457
Reconstituted Complex Homo sapiens
14 WDR37 22884
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 IQCF1  
Affinity Capture-MS Homo sapiens
16 LYN 4067
Proximity Label-MS Homo sapiens
17 MCAM 4162
Proximity Label-MS Homo sapiens
18 NTRK1 4914
Affinity Capture-MS Homo sapiens
19 C17orf70  
Affinity Capture-MS Homo sapiens
20 EPHA2 1969
Proximity Label-MS Homo sapiens
21 CCDC101  
Affinity Capture-MS Homo sapiens
22 PPP1R7 5510
Affinity Capture-MS Homo sapiens
23 YWHAG 7532
Affinity Capture-MS Homo sapiens
24 SPANXN2  
Affinity Capture-MS Homo sapiens
25 SLC22A4 6583
Affinity Capture-MS Homo sapiens
26 CTDSPL 10217
Proximity Label-MS Homo sapiens
27 LEAP2  
Affinity Capture-MS Homo sapiens
28 FGFR1OP  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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