Gene description for DOLK
Gene name dolichol kinase
Gene symbol DOLK
Other names/aliases CDG1M
DK
DK1
SEC59
TMEM15
Species Homo sapiens
 Database cross references - DOLK
ExoCarta ExoCarta_22845
Vesiclepedia VP_22845
Entrez Gene 22845
HGNC 23406
MIM 610746
UniProt Q9UPQ8  
 DOLK identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DOLK
Molecular Function
    dolichol kinase activity GO:0004168 IBA
    dolichol kinase activity GO:0004168 IDA
    dolichol kinase activity GO:0004168 IMP
    dolichol kinase activity GO:0004168 TAS
    protein binding GO:0005515 IPI
Biological Process
    protein N-linked glycosylation GO:0006487 IGI
    dolichyl diphosphate biosynthetic process GO:0006489 TAS
    dolichol metabolic process GO:0019348 IGI
    protein mannosylation GO:0035268 IMP
    dolichyl monophosphate biosynthetic process GO:0043048 IBA
    dolichyl monophosphate biosynthetic process GO:0043048 IDA
    dolichyl monophosphate biosynthetic process GO:0043048 IMP
    dolichol phosphate mannose biosynthetic process GO:0180047 IGI
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified DOLK in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DOLK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SLC2A12 154091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 GJA8  
Two-hybrid Homo sapiens
5 KCNA3  
Two-hybrid Homo sapiens
6 GPX8 493869
Two-hybrid Homo sapiens
7 FNDC9  
Two-hybrid Homo sapiens
8 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 KCNA2  
Affinity Capture-MS Homo sapiens
10 SYNPR  
Two-hybrid Homo sapiens
11 GPR17 2840
Affinity Capture-MS Homo sapiens
12 BTNL9  
Affinity Capture-MS Homo sapiens
13 OPALIN  
Affinity Capture-MS Homo sapiens
14 CMKLR1 1240
Affinity Capture-MS Homo sapiens
15 CD274 29126
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ERGIC3 51614
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TMEM63A 9725
Affinity Capture-MS Homo sapiens
19 LRRC4C  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
20 SLC7A14  
Affinity Capture-MS Homo sapiens
21 KCNA1  
Two-hybrid Homo sapiens
22 CD79A  
Two-hybrid Homo sapiens
23 KLRG2 346689
Affinity Capture-MS Homo sapiens
24 CREB3L1  
Two-hybrid Homo sapiens
25 OCIAD1 54940
Affinity Capture-MS Homo sapiens
26 TMEM80  
Two-hybrid Homo sapiens
27 FAM209A 200232
Two-hybrid Homo sapiens
28 SLC18A2  
Affinity Capture-MS Homo sapiens
29 PDE3A  
Affinity Capture-MS Homo sapiens
30 EDA  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 SLC7A6 9057
Two-hybrid Homo sapiens
32 KCNA10  
Two-hybrid Homo sapiens
33 KCNA6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
34 CHODL 140578
Two-hybrid Homo sapiens
35 ATP13A3 79572
Affinity Capture-MS Homo sapiens
36 GALNT8  
Two-hybrid Homo sapiens
37 TMEM45B 120224
Two-hybrid Homo sapiens
38 CREB3  
Two-hybrid Homo sapiens
39 C10orf54 64115
Two-hybrid Homo sapiens
40 KIR2DL4  
Affinity Capture-MS Homo sapiens
41 KRAS 3845
Negative Genetic Homo sapiens
42 CRELD1 78987
Affinity Capture-MS Homo sapiens
43 CCR6  
Affinity Capture-MS Homo sapiens
44 CD63 967
Affinity Capture-MS Homo sapiens
45 SLC22A4 6583
Affinity Capture-MS Homo sapiens
46 TMEM171  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 C1orf95  
Affinity Capture-MS Homo sapiens
48 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 KCNA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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