Gene description for ENG
Gene name endoglin
Gene symbol ENG
Other names/aliases END
HHT1
ORW1
Species Homo sapiens
 Database cross references - ENG
ExoCarta ExoCarta_2022
Entrez Gene 2022
HGNC 3349
MIM 131195
UniProt P17813  
 ENG identified in exosomes derived from the following tissue/cell type
Melanoma cells 22635005    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ENG
Molecular Function
    protein homodimerization activity GO:0042803 IPI
    protein binding GO:0005515 IPI
    type II transforming growth factor beta receptor binding GO:0005114 ISS
    transforming growth factor beta receptor, cytoplasmic mediator activity GO:0005072 IDA
    transforming growth factor beta binding GO:0050431 IPI
    activin binding GO:0048185 TAS
    BMP binding GO:0036122 IPI
    glycosaminoglycan binding GO:0005539 ISS
    transforming growth factor beta-activated receptor activity GO:0005024 IDA
    type I transforming growth factor beta receptor binding GO:0034713 ISS
    galactose binding GO:0005534 IDA
    transmembrane signaling receptor activity GO:0004888 NAS
Biological Process
    extracellular matrix disassembly GO:0022617 IMP
    response to hypoxia GO:0001666 IDA
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 TAS
    intracellular signal transduction GO:0035556 IDA
    extracellular matrix constituent secretion GO:0070278 IEA
    wound healing GO:0042060 IMP
    venous blood vessel morphogenesis GO:0048845 ISS
    regulation of cell adhesion GO:0030155 TAS
    response to transforming growth factor beta GO:0071559 IEA
    positive regulation of protein phosphorylation GO:0001934 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    bone development GO:0060348 IEA
    cell migration GO:0016477 IMP
    patterning of blood vessels GO:0001569 ISS
    positive regulation of BMP signaling pathway GO:0030513 IDA
    cell chemotaxis GO:0060326 IMP
    response to corticosteroid GO:0031960 IEA
    BMP signaling pathway GO:0030509 TAS
    vasculogenesis GO:0001570 IMP
    cell motility GO:0048870 IMP
    negative regulation of endothelial cell proliferation GO:0001937 IMP
    positive regulation of gene expression GO:0010628 IEA
    regulation of transcription, DNA-templated GO:0006355 IMP
    heart looping GO:0001947 ISS
    negative regulation of cell migration GO:0030336 IDA
    smooth muscle tissue development GO:0048745 ISS
    artery morphogenesis GO:0048844 ISS
    cellular response to mechanical stimulus GO:0071260 IEA
    negative regulation of protein autophosphorylation GO:0031953 IDA
    cell adhesion GO:0007155 IEA
    detection of hypoxia GO:0070483 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    positive regulation of collagen biosynthetic process GO:0032967 IEA
    central nervous system vasculogenesis GO:0022009 IMP
    regulation of cell proliferation GO:0042127 TAS
    positive regulation of pathway-restricted SMAD protein phosphorylation GO:0010862 IMP
    regulation of transforming growth factor beta receptor signaling pathway GO:0017015 IDA
    negative regulation of nitric-oxide synthase activity GO:0051001 IMP
    regulation of phosphorylation GO:0042325 TAS
    positive regulation of systemic arterial blood pressure GO:0003084 IMP
    negative regulation of pathway-restricted SMAD protein phosphorylation GO:0060394 IMP
    cell migration involved in endocardial cushion formation GO:0003273 IEA
    chronological cell aging GO:0001300 IEP
    response to statin GO:0036273 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 IDA
Subcellular Localization
    transforming growth factor beta receptor homodimeric complex GO:0070022 IC
    cytoplasm GO:0005737 IDA
    endothelial microparticle GO:0072563 IEA
    nucleoplasm GO:0005654 IDA
    receptor complex GO:0043235 IPI
    extracellular space GO:0005615 IDA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 IDA
    focal adhesion GO:0005925 IDA
 Experiment description of studies that identified ENG in exosomes
1
Experiment ID 200
ISEV standards
EM
EV Biophysical techniques
HSP70|HSP90|TSG101|Alix|GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22635005    
Organism Homo sapiens
Experiment description Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.
Authors Peinado H, Aleckovic M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, Hergueta-Redondo M, Williams C, Garcia-Santos G, Ghajar C, Nitadori-Hoshino A, Hoffman C, Badal K, Garcia BA, Callahan MK, Yuan J, Martins VR, Skog J, Kaplan RN, Brady MS, Wolchok JD, Chapman PB, Kang Y, Bromberg J, Lyden D.
Journal name Nat Med
Publication year 2012
Sample Melanoma cells
Sample name B16-F10
SK-MEL-202
SK-MEL035
SK-MEL-265
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 257
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 259
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 260
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ENG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACVR1 90
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 ACVR2A  
Affinity Capture-Western Homo sapiens
3 BMP2  
Affinity Capture-Western Homo sapiens
4 ACVR2B  
Affinity Capture-Western Homo sapiens
5 TGFBR2 7048
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 TGFB1 7040
Affinity Capture-MS Homo sapiens
7 INHBA 3624
Affinity Capture-MS Homo sapiens
8 TGFBR1  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 BMP7 655
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which ENG is involved
No pathways found





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