Gene description for IRGM
Gene name immunity-related GTPase family, M
Gene symbol IRGM
Other names/aliases IFI1
IRGM1
LRG-47
LRG47
Species Homo sapiens
 Database cross references - IRGM
ExoCarta ExoCarta_345611
Vesiclepedia VP_345611
Entrez Gene 345611
HGNC 29597
MIM 608212
UniProt A1A4Y4  
 IRGM identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for IRGM
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    G protein activity GO:0003925 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    protein kinase binding GO:0019901 IPI
    protein-macromolecule adaptor activity GO:0030674 IDA
    protein serine/threonine kinase activator activity GO:0043539 IDA
    protein serine/threonine kinase activator activity GO:0043539 IMP
    CARD domain binding GO:0050700 IMP
    BH3 domain binding GO:0051434 IMP
    cardiolipin binding GO:1901612 IDA
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 IMP
    positive regulation of protein phosphorylation GO:0001934 IMP
    inflammatory response GO:0006954 IEA
    positive regulation of autophagy GO:0010508 IDA
    positive regulation of autophagy GO:0010508 IMP
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 IMP
    protein destabilization GO:0031648 IMP
    negative regulation of type I interferon production GO:0032480 IDA
    negative regulation of type II interferon production GO:0032689 ISS
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IMP
    cellular response to interferon-beta GO:0035458 IBA
    defense response to bacterium GO:0042742 IDA
    defense response to bacterium GO:0042742 IMP
    positive regulation of macrophage activation GO:0043032 ISS
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IDA
    regulation of protein-containing complex assembly GO:0043254 IMP
    innate immune response GO:0045087 IBA
    innate immune response GO:0045087 IMP
    negative regulation of defense response to virus GO:0050687 IDA
    negative regulation of inflammatory response GO:0050728 IDA
    protein stabilization GO:0050821 IMP
    defense response to Gram-negative bacterium GO:0050829 IMP
    positive regulation of type II interferon-mediated signaling pathway GO:0060335 IMP
    regulation of protein complex stability GO:0061635 IMP
    protein lipidation involved in autophagosome assembly GO:0061739 IMP
    CAMKK-AMPK signaling cascade GO:0061762 IMP
    protein-containing complex assembly GO:0065003 IDA
    nucleotide-binding oligomerization domain containing 2 signaling pathway GO:0070431 IMP
    protein targeting to vacuole involved in autophagy GO:0071211 IDA
    cellular response to lipopolysaccharide GO:0071222 IMP
    positive regulation of protein serine/threonine kinase activity GO:0071902 IMP
    positive regulation of mitochondrial fission GO:0090141 IDA
    autophagosome maturation GO:0097352 ISS
    cellular response to virus GO:0098586 IMP
    negative regulation of cGAS/STING signaling pathway GO:0160049 IDA
    negative regulation of NLRP3 inflammasome complex assembly GO:1900226 IDA
    positive regulation of autophagosome maturation GO:1901098 IMP
    positive regulation of mitophagy GO:1901526 IDA
    positive regulation of xenophagy GO:1904417 IDA
    positive regulation of lysosome organization GO:1905673 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    autophagosome membrane GO:0000421 IDA
    phagocytic cup GO:0001891 IEA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IDA
    lysosomal membrane GO:0005765 ISS
    endoplasmic reticulum membrane GO:0005789 IBA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    phagocytic vesicle membrane GO:0030670 IEA
    late endosome membrane GO:0031902 IEA
    mitochondrial membrane GO:0031966 ISS
    cell projection GO:0042995 IEA
 Experiment description of studies that identified IRGM in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for IRGM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NOD2 64127
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 ATG16L1 55054
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 CAMKK1  
Affinity Capture-MS Homo sapiens
4 ULK1  
Affinity Capture-Western Homo sapiens
5 IRGM 345611
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 RALGAPB 57148
Affinity Capture-MS Homo sapiens
7 HOXD13  
Affinity Capture-MS Homo sapiens
8 BECN1 8678
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 NOD1 10392
Affinity Capture-Western Homo sapiens
10 TLR3  
Affinity Capture-Western Homo sapiens
11 AMBRA1  
Affinity Capture-Western Homo sapiens
12 ATG14  
Affinity Capture-Western Homo sapiens
13 DDX58 23586
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which IRGM is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here