Gene description for ABCA1
Gene name ATP-binding cassette, sub-family A (ABC1), member 1
Gene symbol ABCA1
Other names/aliases ABC-1
ABC1
CERP
HDLDT1
TGD
Species Homo sapiens
 Database cross references - ABCA1
ExoCarta ExoCarta_19
Vesiclepedia VP_19
Entrez Gene 19
HGNC 29
MIM 600046
UniProt O95477  
 ABCA1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ABCA1
Molecular Function
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    phospholipid transporter activity GO:0005548 IGI
    high-density lipoprotein particle binding GO:0008035 IEA
    protein transmembrane transporter activity GO:0008320 ISS
    cholesterol binding GO:0015485 IC
    ATP hydrolysis activity GO:0016887 IEA
    syntaxin binding GO:0019905 IPI
    phosphatidylcholine binding GO:0031210 IDA
    small GTPase binding GO:0031267 IPI
    apolipoprotein binding GO:0034185 IPI
    apolipoprotein A-I binding GO:0034186 IPI
    apolipoprotein A-I receptor activity GO:0034188 IDA
    ATPase-coupled transmembrane transporter activity GO:0042626 IBA
    sphingolipid floppase activity GO:0046623 IDA
    ATPase binding GO:0051117 IPI
    phosphatidylcholine floppase activity GO:0090554 IBA
    phosphatidylcholine floppase activity GO:0090554 IDA
    phosphatidylcholine floppase activity GO:0090554 IMP
    phosphatidylserine floppase activity GO:0090556 IBA
    phosphatidylserine floppase activity GO:0090556 IDA
    cholesterol transfer activity GO:0120020 IDA
    floppase activity GO:0140328 IDA
    ABC-type transporter activity GO:0140359 IEA
Biological Process
    peptide secretion GO:0002790 IEA
    phagocytosis, engulfment GO:0006911 IEA
    lysosome organization GO:0007040 IDA
    G protein-coupled receptor signaling pathway GO:0007186 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IMP
    cholesterol metabolic process GO:0008203 IDA
    protein secretion GO:0009306 IMP
    protein secretion GO:0009306 ISS
    negative regulation of macrophage derived foam cell differentiation GO:0010745 TAS
    positive regulation of cholesterol efflux GO:0010875 IEA
    negative regulation of cholesterol storage GO:0010887 TAS
    endosomal transport GO:0016197 IDA
    signal release GO:0023061 IMP
    signal release GO:0023061 ISS
    intracellular cholesterol transport GO:0032367 IMP
    regulation of Cdc42 protein signal transduction GO:0032489 IMP
    cholesterol efflux GO:0033344 IDA
    cholesterol efflux GO:0033344 IGI
    cholesterol efflux GO:0033344 IMP
    response to vitamin B3 GO:0033552 IEA
    phospholipid efflux GO:0033700 IBA
    phospholipid efflux GO:0033700 IDA
    phospholipid efflux GO:0033700 IGI
    phospholipid efflux GO:0033700 IMP
    high-density lipoprotein particle assembly GO:0034380 IMP
    response to laminar fluid shear stress GO:0034616 IDA
    apolipoprotein A-I-mediated signaling pathway GO:0038027 IEA
    lipoprotein biosynthetic process GO:0042158 IEA
    cholesterol homeostasis GO:0042632 IDA
    cholesterol homeostasis GO:0042632 TAS
    reverse cholesterol transport GO:0043691 IMP
    phospholipid translocation GO:0045332 IDA
    phospholipid homeostasis GO:0055091 IMP
    platelet dense granule organization GO:0060155 IMP
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to retinoic acid GO:0071300 IEA
    cellular response to cytokine stimulus GO:0071345 IEA
    cellular response to cholesterol GO:0071397 IEA
    cellular response to low-density lipoprotein particle stimulus GO:0071404 NAS
    cellular response to xenobiotic stimulus GO:0071466 IEA
    protein transmembrane transport GO:0071806 ISS
    regulation of high-density lipoprotein particle assembly GO:0090107 TAS
    positive regulation of high-density lipoprotein particle assembly GO:0090108 ISS
    sphingolipid translocation GO:0099039 IEA
    export across plasma membrane GO:0140115 ISS
Subcellular Localization
    endosome GO:0005768 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    Golgi apparatus GO:0005794 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IEA
    basolateral plasma membrane GO:0016323 IEA
    endocytic vesicle GO:0030139 IDA
    intracellular membrane-bounded organelle GO:0043231 IBA
    membrane raft GO:0045121 IDA
    phagocytic vesicle GO:0045335 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    intracellular vesicle GO:0097708 IDA
 Experiment description of studies that identified ABCA1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ABCA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RELA 5970
Affinity Capture-MS Homo sapiens
2 FADD 8772
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 COPS2 9318
Affinity Capture-Western Homo sapiens
4 STX12 23673
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 UGP2 7360
Two-hybrid Homo sapiens
6 AOX1 316
Two-hybrid Homo sapiens
7 IKBKG 8517
Two-hybrid Homo sapiens
8 SPTLC1 10558
Affinity Capture-Western Homo sapiens
9 PRPF8 10594
Two-hybrid Homo sapiens
10 EGFR 1956
Affinity Capture-MS Homo sapiens
11 TGM2 7052
Two-hybrid Homo sapiens
12 PARK2  
Affinity Capture-MS Homo sapiens
13 FUS 2521
Affinity Capture-MS Homo sapiens
14 CDC42 998
Affinity Capture-Western Homo sapiens
15 ATXN10 25814
Two-hybrid Homo sapiens
16 MYC  
Affinity Capture-MS Homo sapiens
17 SDHB 6390
Two-hybrid Homo sapiens
18 CSNK2A2 1459
Biochemical Activity Homo sapiens
19 COPS5 10987
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 HGS 9146
Affinity Capture-Western Homo sapiens
21 SNTB2 6645
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
22 NR1H2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 LNX1  
Biochemical Activity Homo sapiens
24 FLOT1 10211
Affinity Capture-Western Homo sapiens
25 APOA1 335
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
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