Gene description for NECAB3
Gene name N-terminal EF-hand calcium binding protein 3
Gene symbol NECAB3
Other names/aliases APBA2BP
EFCBP3
NIP1
STIP3
SYTIP2
XB51
dJ63M2.4
dJ63M2.5
Species Homo sapiens
 Database cross references - NECAB3
ExoCarta ExoCarta_63941
Entrez Gene 63941
HGNC 15851
MIM 612478
UniProt Q96P71  
 NECAB3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for NECAB3
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    regulation of amyloid precursor protein biosynthetic process GO:0042984 IDA
    protein secretion GO:0009306 NAS
    protein metabolic process GO:0019538 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    Golgi cis cisterna GO:0000137 IDA
    nucleus GO:0005634 NAS
 Experiment description of studies that identified NECAB3 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NECAB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APBA2 321
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which NECAB3 is involved
No pathways found





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