Gene description for C8G
Gene name complement component 8, gamma polypeptide
Gene symbol C8G
Other names/aliases C8C
Species Homo sapiens
 Database cross references - C8G
ExoCarta ExoCarta_733
Vesiclepedia VP_733
Entrez Gene 733
HGNC 1354
MIM 120930
UniProt P07360  
 C8G identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for C8G
Molecular Function
    protein binding GO:0005515 IPI
    retinol binding GO:0019841 IEA
    protein-containing complex binding GO:0044877 IEA
Biological Process
    complement activation, alternative pathway GO:0006957 IEA
    complement activation, classical pathway GO:0006958 IEA
    killing of cells of another organism GO:0031640 IEA
    positive regulation of immune response GO:0050778 NAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    membrane attack complex GO:0005579 IPI
    plasma membrane GO:0005886 NAS
    extracellular exosome GO:0070062 HDA
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified C8G in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for C8G
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SH3GL1 6455
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 EDEM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 SEC24B 10427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 UBQLN2 29978
Two-hybrid Homo sapiens
5 C8A 731
Reconstituted Complex Homo sapiens
6 RHBDD1 84236
Affinity Capture-MS Homo sapiens
7 TMEM2 23670
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 AGPAT1 10554
Affinity Capture-MS Homo sapiens
9 S1PR2 9294
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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