Gene description for SERPINB8
Gene name serpin peptidase inhibitor, clade B (ovalbumin), member 8
Gene symbol SERPINB8
Other names/aliases CAP2
PI8
Species Homo sapiens
 Database cross references - SERPINB8
ExoCarta ExoCarta_5271
Vesiclepedia VP_5271
Entrez Gene 5271
HGNC 8952
MIM 601697
UniProt P50452  
 SERPINB8 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for SERPINB8
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IBA
    serine-type endopeptidase inhibitor activity GO:0004867 IDA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of endopeptidase activity GO:0010951 IBA
    negative regulation of endopeptidase activity GO:0010951 IDA
    epithelial cell-cell adhesion GO:0090136 IMP
Subcellular Localization
    extracellular space GO:0005615 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SERPINB8 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SERPINB8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 F10 2159
Reconstituted Complex Homo sapiens
2 TTC5  
Co-fractionation Homo sapiens
3 SIX6  
Affinity Capture-MS Homo sapiens
4 SPICE1  
Affinity Capture-MS Homo sapiens
5 CR1L 1379
Affinity Capture-MS Homo sapiens
6 IFNA4  
Affinity Capture-MS Homo sapiens
7 SARAF  
Affinity Capture-MS Homo sapiens
8 OR13C3  
Affinity Capture-MS Homo sapiens
9 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
10 ITPA 3704
Co-fractionation Homo sapiens
11 MAP7 9053
Affinity Capture-MS Homo sapiens
12 SEP15 9403
Affinity Capture-MS Homo sapiens
13 NHLH2  
Affinity Capture-MS Homo sapiens
14 NDUFS5 4725
Affinity Capture-MS Homo sapiens
15 ZNF675  
Affinity Capture-MS Homo sapiens
16 TSPAN6 7105
Affinity Capture-MS Homo sapiens
17 POLR2H 5437
Affinity Capture-MS Homo sapiens
18 FCF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 POLR2L 5441
Affinity Capture-MS Homo sapiens
20 PRR19  
Affinity Capture-MS Homo sapiens
21 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 MOGAT3  
Affinity Capture-MS Homo sapiens
23 SERPINB6 5269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 WDR33 55339
Affinity Capture-MS Homo sapiens
25 VAMP4 8674
Affinity Capture-MS Homo sapiens
26 PRDM12  
Affinity Capture-MS Homo sapiens
27 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
28 CXCL14  
Affinity Capture-MS Homo sapiens
29 CDK15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 C1orf35  
Affinity Capture-MS Homo sapiens
31 ZNF713  
Affinity Capture-MS Homo sapiens
32 CDKL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ACYP1 97
Co-fractionation Homo sapiens
34 RINL  
Affinity Capture-MS Homo sapiens
35 ANKRD39  
Affinity Capture-MS Homo sapiens
36 ABCA9 10350
Affinity Capture-MS Homo sapiens
37 ATN1  
Affinity Capture-MS Homo sapiens
38 DDX58 23586
Affinity Capture-RNA Homo sapiens
39 EMX2  
Affinity Capture-MS Homo sapiens
40 ZIC1  
Affinity Capture-MS Homo sapiens
41 ZP3 7784
Affinity Capture-MS Homo sapiens
42 NONO 4841
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 C21orf59 56683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 IMPA2 3613
Co-fractionation Homo sapiens
45 TAS2R8  
Affinity Capture-MS Homo sapiens
46 ASCL1  
Affinity Capture-MS Homo sapiens
47 HES4  
Affinity Capture-MS Homo sapiens
48 GPATCH2L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 REG3A  
Affinity Capture-MS Homo sapiens
50 PRSS16 10279
Affinity Capture-MS Homo sapiens
51 BCL2  
Affinity Capture-Western Homo sapiens
52 PLXDC2 84898
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 AMTN  
Affinity Capture-MS Homo sapiens
54 ZC3HC1  
Affinity Capture-MS Homo sapiens
55 MCM2 4171
Affinity Capture-MS Homo sapiens
56 CASP3 836
Co-fractionation Homo sapiens
57 COA5  
Affinity Capture-MS Homo sapiens
58 GOT1 2805
Affinity Capture-MS Homo sapiens
59 TOX4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 SSUH2  
Affinity Capture-MS Homo sapiens
61 PRSS1 5644
Reconstituted Complex Homo sapiens
62 LARS 51520
Co-fractionation Homo sapiens
63 UGT1A3  
Affinity Capture-MS Homo sapiens
64 OR2A4 79541
Affinity Capture-MS Homo sapiens
65 ZRSR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 DCDC5  
Affinity Capture-MS Homo sapiens
67 ARMT1 79624
Co-fractionation Homo sapiens
68 CTH 1491
Co-fractionation Homo sapiens
69 CCR1  
Affinity Capture-MS Homo sapiens
70 OR2H2  
Affinity Capture-MS Homo sapiens
71 FAM102A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 STX17 55014
Affinity Capture-MS Homo sapiens
73 RIPPLY3  
Affinity Capture-MS Homo sapiens
74 SOX2  
Affinity Capture-MS Homo sapiens
75 TRIM43  
Affinity Capture-MS Homo sapiens
76 FNDC5  
Affinity Capture-MS Homo sapiens
77 FURIN 5045
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which SERPINB8 is involved
PathwayEvidenceSource
Dissolution of Fibrin Clot TAS Reactome
Hemostasis TAS Reactome





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