ExoCarta: Gene summary

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Gene description for PRSS2
Gene name	protease, serine, 2 (trypsin 2)
Gene symbol	PRSS2
Other names/aliases	TRY2 TRY8 TRYP2
Species	Homo sapiens
Database cross references - PRSS2
ExoCarta	ExoCarta_5645
Vesiclepedia	VP_5645
Entrez Gene	5645
HGNC	9483
MIM	601564
UniProt	P07478
PRSS2 identified in sEVs derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686
Chondrocytes	35931686
Chondrocytes	35931686

Gene ontology annotations for PRSS2

Molecular Function
	metalloendopeptidase activity	GO:0004222	TAS
	serine-type endopeptidase activity	GO:0004252	IBA
	serine-type endopeptidase activity	GO:0004252	IDA
	serine-type endopeptidase activity	GO:0004252	TAS
	calcium ion binding	GO:0005509	IDA
	protein binding	GO:0005515	IPI
	serine-type peptidase activity	GO:0008236	TAS
Biological Process
	proteolysis	GO:0006508	IBA
	proteolysis	GO:0006508	IDA
	digestion	GO:0007586	IEA
	antimicrobial humoral response	GO:0019730	TAS
	extracellular matrix disassembly	GO:0022617	TAS
	positive regulation of cell growth	GO:0030307	TAS
	collagen catabolic process	GO:0030574	IDA
	collagen catabolic process	GO:0030574	IMP
	collagen catabolic process	GO:0030574	TAS
	positive regulation of cell adhesion	GO:0045785	TAS
Subcellular Localization
	extracellular region	GO:0005576	IDA
	extracellular region	GO:0005576	TAS
	extracellular space	GO:0005615	IBA
	extracellular space	GO:0005615	IMP
	extracellular matrix	GO:0031012	TAS
	azurophil granule lumen	GO:0035578	TAS

Experiment description of studies that identified PRSS2 in sEVs

Experiment ID

494

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD151\|CD63\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Osteoarthritic cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

496

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Healthy cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

497

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

498

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for PRSS2

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

PYGO1

Affinity Capture-MS

Homo sapiens

HSPA5

3309

Affinity Capture-MS

Homo sapiens

HOXA2

Proximity Label-MS

Homo sapiens

SLC35A2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CD8B

926

Affinity Capture-MS

Homo sapiens

TUBB3

10381

Affinity Capture-MS

Homo sapiens

RIN3

Affinity Capture-MS

Homo sapiens

METRN

79006

Affinity Capture-MS

Homo sapiens

PRSS3

5646

Affinity Capture-MS

Homo sapiens

GLP1R

PCA

Homo sapiens

KIF20A

10112

Affinity Capture-MS

Homo sapiens

USP11

8237

Affinity Capture-MS

Homo sapiens

FUBP3

8939

Affinity Capture-MS

Homo sapiens

TUBB1

81027

Affinity Capture-MS

Homo sapiens

SBDS

51119

Affinity Capture-MS

Homo sapiens

FN1

2335

Affinity Capture-MS

Homo sapiens

SYDE1

85360

Affinity Capture-MS

Homo sapiens

POTEF

728378

Affinity Capture-MS

Homo sapiens

FCER1A

Affinity Capture-MS

Homo sapiens

BTBD1

Affinity Capture-MS

Homo sapiens

CSN1S1

1446

Biochemical Activity

Homo sapiens

GDF11

10220

Affinity Capture-MS

Homo sapiens

CIDEC

Affinity Capture-MS

Homo sapiens

PRSS1

5644

Affinity Capture-MS

Homo sapiens

SUMF1

285362

Affinity Capture-MS

Homo sapiens

GSTT1

2952

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TMEM252

Affinity Capture-MS

Homo sapiens

BMP1

649

Affinity Capture-MS

Homo sapiens

ARSK

Affinity Capture-MS

Homo sapiens

DKK2

Affinity Capture-MS

Homo sapiens

ITGA4

3676

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which PRSS2 is involved

Pathway	Evidence	Source
Activation of Matrix Metalloproteinases	TAS	Reactome
Alpha-defensins	TAS	Reactome
Antimicrobial peptides	TAS	Reactome
Collagen degradation	TAS	Reactome
Defensins	TAS	Reactome
Degradation of the extracellular matrix	TAS	Reactome
Extracellular matrix organization	TAS	Reactome
Immune System	TAS	Reactome
Innate Immune System	TAS	Reactome
Neutrophil degranulation	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here