Gene description for SPTBN4
Gene name spectrin, beta, non-erythrocytic 4
Gene symbol SPTBN4
Other names/aliases QV
SPNB4
SPTBN3
Species Homo sapiens
 Database cross references - SPTBN4
ExoCarta ExoCarta_57731
Entrez Gene 57731
HGNC 14896
MIM 606214
UniProt Q9H254  
 SPTBN4 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SPTBN4
Molecular Function
    ankyrin binding GO:0030506 ISS
    phospholipid binding GO:0005543 IEA
    protein binding GO:0005515 IPI
    structural constituent of cytoskeleton GO:0005200 TAS
    phosphatase binding GO:0019902 IPI
    spectrin binding GO:0030507 ISS
    actin binding GO:0003779 TAS
Biological Process
    fertilization GO:0009566 IEA
    clustering of voltage-gated sodium channels GO:0045162 ISS
    central nervous system projection neuron axonogenesis GO:0021952 ISS
    axonogenesis GO:0007409 ISS
    transmission of nerve impulse GO:0019226 ISS
    negative regulation of heart rate GO:0010459 ISS
    vesicle-mediated transport GO:0016192 TAS
    regulation of peptidyl-serine phosphorylation GO:0033135 ISS
    establishment of protein localization to plasma membrane GO:0090002 ISS
    actin filament capping GO:0051693 IEA
    axon guidance GO:0007411 TAS
    cytoskeletal anchoring at plasma membrane GO:0007016 TAS
    adult walking behavior GO:0007628 ISS
    sensory perception of sound GO:0007605 ISS
    positive regulation of multicellular organism growth GO:0040018 IEA
    regulation of sodium ion transport GO:0002028 ISS
    cardiac conduction GO:0061337 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISS
    axon hillock GO:0043203 ISS
    plasma membrane GO:0005886 ISS
    cell body fiber GO:0070852 ISS
    nuclear matrix GO:0016363 ISS
    extracellular exosome GO:0070062 IDA
    node of Ranvier GO:0033268 ISS
    membrane GO:0016020 IDA
    axon initial segment GO:0043194 ISS
    spectrin GO:0008091 ISS
    PML body GO:0016605 ISS
    cytosol GO:0005829 TAS
    adherens junction GO:0005912 ISS
    neuronal cell body GO:0043025 ISS
    paranode region of axon GO:0033270 IEA
 Experiment description of studies that identified SPTBN4 in exosomes
1
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
3
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPTBN4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRN  
Invivo Homo sapiens
Two-hybrid Homo sapiens
2 ANK3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 PTPRN2  
Invivo Homo sapiens
Two-hybrid Homo sapiens
4 DISC1 27185
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SPTBN4 is involved
PathwayEvidenceSource
Interaction between L1 and Ankyrins TAS Reactome
NCAM signaling for neurite out-growth TAS Reactome





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