Gene description for RHCG
Gene name Rh family, C glycoprotein
Gene symbol RHCG
Other names/aliases C15orf6
PDRC2
RHGK
SLC42A3
Species Homo sapiens
 Database cross references - RHCG
ExoCarta ExoCarta_51458
Vesiclepedia VP_51458
Entrez Gene 51458
HGNC 18140
MIM 605381
UniProt Q9UBD6  
 RHCG identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for RHCG
Molecular Function
    protein binding GO:0005515 IPI
    ammonium channel activity GO:0008519 IBA
    ammonium channel activity GO:0008519 IDA
    ammonium channel activity GO:0008519 IGI
    ammonium channel activity GO:0008519 TAS
    ankyrin binding GO:0030506 IDA
    carbon dioxide transmembrane transporter activity GO:0035379 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    intracellular monoatomic ion homeostasis GO:0006873 IDA
    regulation of pH GO:0006885 IEA
    amine transport GO:0015837 NAS
    epithelial cell differentiation GO:0030855 NAS
    carbon dioxide transmembrane transport GO:0035378 IEA
    homeostatic process GO:0042592 NAS
    transepithelial ammonium transport GO:0070634 IDA
    ammonium transmembrane transport GO:0072488 IBA
    ammonium transmembrane transport GO:0072488 IDA
    ammonium transmembrane transport GO:0072488 IGI
    ammonium homeostasis GO:0097272 IBA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    cytoplasmic vesicle GO:0031410 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified RHCG in exosomes
1
Experiment ID 13
MISEV standards
IEM
Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RHCG
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SSUH2  
Affinity Capture-MS Homo sapiens
2 TMEM60  
Two-hybrid Homo sapiens
3 CXCL9  
Two-hybrid Homo sapiens
4 EFNA5  
Two-hybrid Homo sapiens
5 GPR25  
Two-hybrid Homo sapiens
6 UPK1B 7348
Two-hybrid Homo sapiens
7 TUSC5  
Two-hybrid Homo sapiens
8 FXYD6  
Two-hybrid Homo sapiens
9 IGFBP5 3488
Two-hybrid Homo sapiens
10 C1QL4  
Two-hybrid Homo sapiens
11 MS4A13  
Two-hybrid Homo sapiens
12 SCARB2 950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TMEM97 27346
Two-hybrid Homo sapiens
14 YIPF6 286451
Two-hybrid Homo sapiens
15 C4orf3  
Two-hybrid Homo sapiens
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