Gene description for RHCG
Gene name Rh family, C glycoprotein
Gene symbol RHCG
Other names/aliases C15orf6
PDRC2
RHGK
SLC42A3
Species Homo sapiens
 Database cross references - RHCG
ExoCarta ExoCarta_51458
Entrez Gene 51458
HGNC 18140
MIM 605381
UniProt Q9UBD6  
 RHCG identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for RHCG
Molecular Function
    ammonium transmembrane transporter activity GO:0008519 IGI
    ankyrin binding GO:0030506 IDA
Biological Process
    amine transport GO:0015837 NAS
    ammonium transport GO:0015696 IGI
    cellular ion homeostasis GO:0006873 IDA
    epithelial cell differentiation GO:0030855 NAS
    transepithelial ammonium transport GO:0070634 IDA
    homeostatic process GO:0042592 NAS
    regulation of pH GO:0006885 IEA
    transmembrane transport GO:0055085 TAS
    ammonium transmembrane transport GO:0072488 IGI
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    cytoplasmic vesicle GO:0031410 ISS
    basolateral plasma membrane GO:0016323 IDA
    plasma membrane GO:0005886 TAS
    integral component of plasma membrane GO:0005887 IDA
    apical plasma membrane GO:0016324 IDA
 Experiment description of studies that identified RHCG in exosomes
1
Experiment ID 13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RHCG
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RHCG is involved
PathwayEvidenceSource
Rhesus glycoproteins mediate ammonium transport. TAS Reactome





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