Gene description for SLC12A3
Gene name solute carrier family 12 (sodium/chloride transporter), member 3
Gene symbol SLC12A3
Other names/aliases NCC
NCCT
TSC
Species Homo sapiens
 Database cross references - SLC12A3
ExoCarta ExoCarta_6559
Vesiclepedia VP_6559
Entrez Gene 6559
HGNC 10912
MIM 600968
UniProt P55017  
 SLC12A3 identified in sEVs derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
Urine 21082674    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for SLC12A3
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    sodium:potassium:chloride symporter activity GO:0008511 IBA
    sodium:chloride symporter activity GO:0015378 IDA
    sodium:chloride symporter activity GO:0015378 TAS
Biological Process
    response to dietary excess GO:0002021 IEA
    monoatomic ion transport GO:0006811 TAS
    sodium ion transport GO:0006814 ISS
    cell volume homeostasis GO:0006884 IBA
    signal transduction GO:0007165 IEA
    sodium ion transmembrane transport GO:0035725 IBA
    sodium ion transmembrane transport GO:0035725 ISS
    chloride ion homeostasis GO:0055064 IBA
    potassium ion homeostasis GO:0055075 IBA
    sodium ion homeostasis GO:0055078 IBA
    renal sodium ion absorption GO:0070294 IDA
    chloride transmembrane transport GO:1902476 IBA
    response to aldosterone GO:1904044 IEA
    potassium ion import across plasma membrane GO:1990573 IBA
Subcellular Localization
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISS
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified SLC12A3 in sEVs
1
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 102
MISEV standards
✔
IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21082674    
Organism Homo sapiens
Experiment description "Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS."
Authors "Zhang Y, Li Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Immunoelectron Microscopy
4
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SLC12A3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 SNX1 6642
Affinity Capture-MS Homo sapiens
3 SNX2 6643
Affinity Capture-MS Homo sapiens
4 Bmpr1a  
Affinity Capture-MS Mus musculus
5 DDRGK1 65992
Affinity Capture-MS Homo sapiens
6 NEDD4 4734
Affinity Capture-Western Homo sapiens
7 PDIA3 2923
Two-hybrid Homo sapiens
8 KLHL3  
Affinity Capture-Western Homo sapiens
9 GUCY1B3  
Affinity Capture-MS Homo sapiens
10 SNX5 27131
Affinity Capture-MS Homo sapiens
11 HSPA8 3312
Affinity Capture-MS Homo sapiens
12 SNX6 58533
Affinity Capture-MS Homo sapiens
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