Gene description for WLS
Gene name wntless Wnt ligand secretion mediator
Gene symbol WLS
Other names/aliases C1orf139
EVI
GPR177
MRP
mig-14
Species Homo sapiens
 Database cross references - WLS
ExoCarta ExoCarta_79971
Vesiclepedia VP_79971
Entrez Gene 79971
HGNC 30238
MIM 611514
UniProt Q5T9L3  
 WLS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for WLS
Molecular Function
    protein binding GO:0005515 IPI
    Wnt-protein binding GO:0017147 IBA
    Wnt-protein binding GO:0017147 IPI
    Wnt-protein binding GO:0017147 ISS
    identical protein binding GO:0042802 IPI
Biological Process
    mesoderm formation GO:0001707 IEA
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 IMP
    anterior/posterior axis specification GO:0009948 ISS
    Wnt signaling pathway GO:0016055 IEA
    positive regulation of Wnt signaling pathway GO:0030177 IMP
    midbrain development GO:0030901 IEA
    hindbrain development GO:0030902 IEA
    exocrine pancreas development GO:0031017 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISS
    Wnt protein secretion GO:0061355 IBA
    Wnt protein secretion GO:0061355 IMP
    positive regulation of Wnt protein secretion GO:0061357 IMP
    positive regulation of Wnt protein secretion GO:0061357 ISS
    cementum mineralization GO:0071529 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 TAS
    early endosome GO:0005769 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    endomembrane system GO:0012505 IBA
    endocytic vesicle membrane GO:0030666 TAS
    organelle membrane GO:0031090 IBA
    cytoplasmic vesicle GO:0031410 IDA
    early endosome membrane GO:0031901 TAS
    extracellular exosome GO:0070062 TAS
 Experiment description of studies that identified WLS in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for WLS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HRH1  
Two-hybrid Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MCAM 4162
Proximity Label-MS Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 RAB35 11021
Proximity Label-MS Homo sapiens
7 TMED10 10972
Affinity Capture-MS Homo sapiens
8 PARP1 142
Proximity Label-MS Homo sapiens
9 NCSTN 23385
Affinity Capture-MS Homo sapiens
10 RPN2 6185
Proximity Label-MS Homo sapiens
11 CANX 821
Affinity Capture-MS Homo sapiens
12 LAMTOR1 55004
Proximity Label-MS Homo sapiens
13 SIDT2 51092
Affinity Capture-MS Homo sapiens
14 LAMP3  
Proximity Label-MS Homo sapiens
15 LRRC59 55379
Proximity Label-MS Homo sapiens
16 DUOXA2  
Affinity Capture-MS Homo sapiens
17 GABRA3  
Affinity Capture-MS Homo sapiens
18 KIAA1715 80856
Proximity Label-MS Homo sapiens
19 OPRK1  
Affinity Capture-Western Homo sapiens
20 RNF128  
Affinity Capture-MS Homo sapiens
21 SMO 6608
Two-hybrid Homo sapiens
22 WNT7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GPR182  
Affinity Capture-MS Homo sapiens
25 WNT4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TMED6  
Affinity Capture-MS Homo sapiens
27 GOLPH3 64083
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 OPRD1  
Affinity Capture-Western Homo sapiens
29 FCER1A  
Affinity Capture-MS Homo sapiens
30 ARF6 382
Proximity Label-MS Homo sapiens
31 CLEC4E  
Affinity Capture-MS Homo sapiens
32 STX4 6810
Proximity Label-MS Homo sapiens
33 FFAR1  
Affinity Capture-MS Homo sapiens
34 SLC22A9  
Affinity Capture-MS Homo sapiens
35 RPN1 6184
Proximity Label-MS Homo sapiens
36 NXF1 10482
Affinity Capture-RNA Homo sapiens
37 Cdc16  
Affinity Capture-MS Mus musculus
38 RAB4A 5867
Proximity Label-MS Homo sapiens
39 RAB5A 5868
Proximity Label-MS Homo sapiens
40 SRP9 6726
Cross-Linking-MS (XL-MS) Homo sapiens
41 MOV10 4343
Affinity Capture-RNA Homo sapiens
42 CGRRF1  
Affinity Capture-MS Homo sapiens
43 SEC62 7095
Proximity Label-MS Homo sapiens
44 ATP2A1 487
Proximity Label-MS Homo sapiens
45 RPRM  
Affinity Capture-MS Homo sapiens
46 WNT3A 89780
Affinity Capture-MS Homo sapiens
47 RAB9A 9367
Proximity Label-MS Homo sapiens
48 CCR2  
Two-hybrid Homo sapiens
49 WNT5A 7474
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 MARCH4  
Affinity Capture-MS Homo sapiens
51 RAB2A 5862
Proximity Label-MS Homo sapiens
52 OPRM1 4988
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
53 DHFRL1  
Proximity Label-MS Homo sapiens
54 KRAS 3845
Proximity Label-MS Homo sapiens
55 RAB5C 5878
Proximity Label-MS Homo sapiens
56 RHOB 388
Proximity Label-MS Homo sapiens
57 VIPR1  
Affinity Capture-MS Homo sapiens
58 GPR37  
Two-hybrid Homo sapiens
59 CD63 967
Affinity Capture-MS Homo sapiens
60 CHRNB1  
Affinity Capture-MS Homo sapiens
61 CHRNA4  
Affinity Capture-MS Homo sapiens
62 WNT2  
Affinity Capture-MS Homo sapiens
63 Ncstn 59287
Affinity Capture-MS Mus musculus
64 WNT16  
Affinity Capture-MS Homo sapiens
65 C9orf72  
Affinity Capture-MS Homo sapiens
66 SEC61B 10952
Proximity Label-MS Homo sapiens
67 DRD2  
Two-hybrid Homo sapiens
68 CHRND  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SNX8 29886
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which WLS is involved
PathwayEvidenceSource
Signal Transduction TAS Reactome
Signaling by WNT TAS Reactome
WNT ligand biogenesis and trafficking TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here