Gene description for CTSS
Gene name cathepsin S
Gene symbol CTSS
Other names/aliases -
Species Homo sapiens
 Database cross references - CTSS
ExoCarta ExoCarta_1520
Vesiclepedia VP_1520
Entrez Gene 1520
HGNC 2545
MIM 116845
UniProt P25774  
 CTSS identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
 Gene ontology annotations for CTSS
Molecular Function
    proteoglycan binding GO:0043394 IPI
    collagen binding GO:0005518 IDA
    cysteine-type endopeptidase activity GO:0004197 IDA
    fibronectin binding GO:0001968 IPI
    laminin binding GO:0043236 IDA
Biological Process
    cellular response to thyroid hormone stimulus GO:0097067 IEP
    adaptive immune response GO:0002250 IEP
    toll-like receptor signaling pathway GO:0002224 TAS
    collagen catabolic process GO:0030574 IDA
    immune response GO:0006955 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I GO:0042590 TAS
    response to acidic pH GO:0010447 IDA
    extracellular matrix organization GO:0030198 TAS
    innate immune response GO:0045087 TAS
    proteolysis GO:0006508 IDA
    proteolysis involved in cellular protein catabolic process GO:0051603 IDA
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    antigen processing and presentation of peptide antigen GO:0048002 IDA
    extracellular matrix disassembly GO:0022617 TAS
    positive regulation of cation channel activity GO:2001259 IDA
    protein processing GO:0016485 IDA
    basement membrane disassembly GO:0034769 IDA
    antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent GO:0002480 TAS
    antigen processing and presentation GO:0019882 TAS
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    positive regulation of inflammatory response GO:0050729 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    lysosomal lumen GO:0043202 TAS
    endolysosome lumen GO:0036021 TAS
    lysosome GO:0005764 IDA
    extracellular space GO:0005615 IDA
    membrane GO:0016020 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified CTSS in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for CTSS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CST3 1471
Invitro Homo sapiens
2 SERPINB3 6317
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
3 PLCG1 5335
Invivo Homo sapiens
Invitro Homo sapiens
4 CST5 1473
Reconstituted Complex Homo sapiens
5 SERPINB4 6318
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 KNG1 3827
Invitro Homo sapiens
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