Gene description for SC5D
Gene name sterol-C5-desaturase
Gene symbol SC5D
Other names/aliases ERG3
S5DES
SC5DL
Species Homo sapiens
 Database cross references - SC5D
ExoCarta ExoCarta_6309
Vesiclepedia VP_6309
Entrez Gene 6309
HGNC 10547
MIM 602286
UniProt O75845  
 SC5D identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SC5D
Molecular Function
    C-5 sterol desaturase activity GO:0000248 EXP
    C-5 sterol desaturase activity GO:0000248 IBA
    iron ion binding GO:0005506 IEA
    delta7-sterol 5(6)-desaturase activity GO:0050046 IDA
Biological Process
    cholesterol biosynthetic process via desmosterol GO:0033489 TAS
    cholesterol biosynthetic process via lathosterol GO:0033490 IBA
    cholesterol biosynthetic process via lathosterol GO:0033490 IDA
    cholesterol biosynthetic process via lathosterol GO:0033490 TAS
    negative regulation of ferroptosis GO:0110076 IMP
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 IBA
 Experiment description of studies that identified SC5D in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SC5D
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPRA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 C5AR1  
Affinity Capture-MS Homo sapiens
3 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RXFP4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 GPR12  
Affinity Capture-MS Homo sapiens
7 CMTM5  
Affinity Capture-MS Homo sapiens
8 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 LZTFL1 54585
Affinity Capture-MS Homo sapiens
10 TSPAN15 23555
Affinity Capture-MS Homo sapiens
11 CMKLR1 1240
Affinity Capture-MS Homo sapiens
12 BSCL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 HSD3B7 80270
Proximity Label-MS Homo sapiens
14 TSPAN17 26262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 LCTL  
Affinity Capture-MS Homo sapiens
16 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TBXA2R 6915
Affinity Capture-MS Homo sapiens
18 SREBF2 6721
Positive Genetic Homo sapiens
19 SEC62 7095
Proximity Label-MS Homo sapiens
20 PCDHGC4  
Affinity Capture-MS Homo sapiens
21 P2RY1  
Affinity Capture-MS Homo sapiens
22 GPR182  
Affinity Capture-MS Homo sapiens
23 ATP1B1 481
Affinity Capture-MS Homo sapiens
24 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 VIPR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 TMEM63A 9725
Affinity Capture-MS Homo sapiens
27 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TSPAN10 83882
Affinity Capture-MS Homo sapiens
29 LPAR1 1902
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 DGCR2 9993
Affinity Capture-MS Homo sapiens
31 AVPR2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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