Gene description for AGT
Gene name angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Gene symbol AGT
Other names/aliases ANHU
SERPINA8
Species Homo sapiens
 Database cross references - AGT
ExoCarta ExoCarta_183
Vesiclepedia VP_183
Entrez Gene 183
HGNC 333
MIM 106150
UniProt P01019  
 AGT identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for AGT
Molecular Function
    superoxide-generating NADPH oxidase activator activity GO:0016176 TAS
    type 2 angiotensin receptor binding GO:0031703 IPI
    hormone activity GO:0005179 ISS
    protein binding GO:0005515 IPI
    serine-type endopeptidase inhibitor activity GO:0004867 TAS
    sodium channel regulator activity GO:0017080 IMP
    type 1 angiotensin receptor binding GO:0031702 IPI
    growth factor activity GO:0008083 TAS
Biological Process
    response to salt stress GO:0009651 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    positive regulation of NAD(P)H oxidase activity GO:0033864 TAS
    response to cold GO:0009409 IEA
    positive regulation of fatty acid biosynthetic process GO:0045723 IEA
    ovarian follicle rupture GO:0001543 IEA
    peristalsis GO:0030432 IEA
    regulation of blood pressure GO:0008217 IGI
    positive regulation of superoxide anion generation GO:0032930 IEA
    positive regulation of cholesterol esterification GO:0010873 IDA
    smooth muscle cell differentiation GO:0051145 IEA
    blood vessel remodeling GO:0001974 TAS
    angiotensin-mediated drinking behavior GO:0003051 IEA
    cellular sodium ion homeostasis GO:0006883 IEA
    positive regulation of vasodilation GO:0045909 IEA
    G-protein coupled receptor signaling pathway GO:0007186 IDA
    positive regulation of membrane hyperpolarization GO:1902632 IMP
    positive regulation of gap junction assembly GO:1903598 IGI
    cell-cell signaling GO:0007267 TAS
    cellular lipid metabolic process GO:0044255 TAS
    angiotensin maturation GO:0002003 TAS
    renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure GO:0001999 IEA
    positive regulation of cardiac muscle hypertrophy GO:0010613 ISS
    positive regulation of multicellular organism growth GO:0040018 IEA
    positive regulation of cytokine production GO:0001819 TAS
    positive regulation of endothelial cell migration GO:0010595 IDA
    cell growth involved in cardiac muscle cell development GO:0061049 IEA
    astrocyte activation GO:0048143 IEA
    negative regulation of sodium ion transmembrane transporter activity GO:2000650 TAS
    activation of NF-kappaB-inducing kinase activity GO:0007250 IEA
    positive regulation of activation of JAK2 kinase activity GO:0010535 IMP
    small molecule metabolic process GO:0044281 TAS
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IEA
    positive regulation of organ growth GO:0046622 IEA
    regulation of vasoconstriction GO:0019229 NAS
    vasodilation GO:0042311 IEA
    female pregnancy GO:0007565 IEA
    positive regulation of extracellular matrix constituent secretion GO:0003331 IEA
    cytokine secretion GO:0050663 IEA
    regulation of norepinephrine secretion GO:0014061 IEA
    response to muscle activity involved in regulation of muscle adaptation GO:0014873 ISS
    regulation of cell growth GO:0001558 NAS
    activation of phospholipase C activity GO:0007202 IEA
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IDA
    positive regulation of extrinsic apoptotic signaling pathway GO:2001238 IDA
    regulation of calcium ion transport GO:0051924 IEA
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IC
    regulation of cell proliferation GO:0042127 NAS
    stress-activated MAPK cascade GO:0051403 IEA
    aging GO:0007568 IEA
    extracellular matrix organization GO:0030198 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    catenin import into nucleus GO:0035411 IEA
    regulation of renal output by angiotensin GO:0002019 NAS
    artery smooth muscle contraction GO:0014824 IEA
    negative regulation of endopeptidase activity GO:0010951 IBA
    phospholipase C-activating G-protein coupled receptor signaling pathway GO:0007200 NAS
    negative regulation of angiogenesis GO:0016525 IEA
    positive regulation of reactive oxygen species metabolic process GO:2000379 TAS
    positive regulation of MAPK cascade GO:0043410 IEA
    excretion GO:0007588 IEA
    cellular protein metabolic process GO:0044267 TAS
    negative regulation of cell proliferation GO:0008285 IEA
    kidney development GO:0001822 IMP
    renal system process GO:0003014 IDA
    cellular response to mechanical stimulus GO:0071260 IEA
    fibroblast proliferation GO:0048144 IEA
    ERK1 and ERK2 cascade GO:0070371 IEA
    positive regulation of protein kinase C activity GO:1900020 IEA
    establishment of blood-nerve barrier GO:0008065 IEA
    positive regulation of cellular protein metabolic process GO:0032270 IDA
    nitric oxide mediated signal transduction GO:0007263 TAS
    negative regulation of neurotrophin TRK receptor signaling pathway GO:0051387 IDA
    regulation of transmission of nerve impulse GO:0051969 IEA
    positive regulation of branching involved in ureteric bud morphogenesis GO:0090190 IDA
    positive regulation of protein tyrosine kinase activity GO:0061098 IMP
    positive regulation of renal sodium excretion GO:0035815 IEA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 TAS
    regulation of extracellular matrix assembly GO:1901201 IGI
    regulation of blood vessel size by renin-angiotensin GO:0002034 TAS
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IDA
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    blood vessel development GO:0001568 IEA
    cell-matrix adhesion GO:0007160 IEA
    negative regulation of tissue remodeling GO:0034104 IEA
    positive regulation of inflammatory response GO:0050729 TAS
    negative regulation of cell growth GO:0030308 IEA
    low-density lipoprotein particle remodeling GO:0034374 NAS
    branching involved in ureteric bud morphogenesis GO:0001658 IEA
    smooth muscle cell proliferation GO:0048659 IEA
    regulation of blood volume by renin-angiotensin GO:0002016 NAS
    angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure GO:0001998 IEA
    regulation of cardiac conduction GO:1903779 IGI
    renin-angiotensin regulation of aldosterone production GO:0002018 NAS
    regulation of long-term neuronal synaptic plasticity GO:0048169 IEA
    regulation of renal sodium excretion GO:0035813 NAS
    positive regulation of fibroblast proliferation GO:0048146 ISS
    uterine smooth muscle contraction GO:0070471 IEA
    G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger GO:0007199 TAS
Subcellular Localization
    extracellular space GO:0005615 ISS
    extracellular exosome GO:0070062 IDA
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 IEA
    blood microparticle GO:0072562 IDA
 Experiment description of studies that identified AGT in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 44
MISEV standards
✔
IEM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AGT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRG2  
Affinity Capture-MS Homo sapiens
2 AGTR1  
Affinity Capture-MS Homo sapiens
3 DNPEP 23549
Affinity Capture-MS Homo sapiens
4 EWSR1 2130
Two-hybrid Homo sapiens
View the network image/svg+xml



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