Gene description for PPFIA2
Gene name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2
Gene symbol PPFIA2
Other names/aliases -
Species Homo sapiens
 Database cross references - PPFIA2
ExoCarta ExoCarta_8499
Entrez Gene 8499
HGNC 9246
MIM 603143
UniProt O75334  
 PPFIA2 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Urine 19056867    
 Gene ontology annotations for PPFIA2
Molecular Function
    protein complex binding GO:0032403 IEA
    protein binding GO:0005515 IPI
Biological Process
    cell-matrix adhesion GO:0007160 TAS
    synaptic transmission GO:0007268 TAS
    neurotransmitter secretion GO:0007269 TAS
    glutamate secretion GO:0014047 TAS
Subcellular Localization
    cell surface GO:0009986 IEA
    presynaptic active zone GO:0048786 TAS
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 TAS
 Experiment description of studies that identified PPFIA2 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PPFIA2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPFIBP2 8495
Invitro Homo sapiens
2 PPP2CA 5515
Affinity Capture-MS Homo sapiens
3 PTPRF 5792
Invivo Homo sapiens
Invitro Homo sapiens
4 PPFIA2 8499
Invitro Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
5 CAST  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 PPFIBP1 8496
Invivo Homo sapiens
Invitro Homo sapiens
7 GIT1 28964
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
8 PPFIA1 8500
Invitro Homo sapiens
Invivo Homo sapiens
9 PTPRS 5802
Invitro Homo sapiens
10 PPFIA3 8541
Invitro Homo sapiens
11 PTPRD 5789
Invitro Homo sapiens
12 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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