Gene description for NANOS1
Gene name nanos homolog 1 (Drosophila)
Gene symbol NANOS1
Other names/aliases NOS1
SPGF12
Species Homo sapiens
 Database cross references - NANOS1
ExoCarta ExoCarta_340719
Entrez Gene 340719
HGNC 23044
MIM 608226
UniProt Q8WY41  
 NANOS1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for NANOS1
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    translation repressor activity GO:0030371 ISS
    zinc ion binding GO:0008270 IEA
Biological Process
    positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay GO:1900153 IDA
    cell migration GO:0016477 IDA
    negative regulation of translation GO:0017148 IDA
    epithelial cell migration GO:0010631 IDA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified NANOS1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NANOS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PUM2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NANOS1 is involved
No pathways found





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