Gene description for ST6GAL1
Gene name ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Gene symbol ST6GAL1
Other names/aliases SIAT1
ST6GalI
ST6N
Species Homo sapiens
 Database cross references - ST6GAL1
ExoCarta ExoCarta_6480
Vesiclepedia VP_6480
Entrez Gene 6480
HGNC 10860
MIM 109675
UniProt P15907  
 ST6GAL1 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ST6GAL1
Molecular Function
    protein binding GO:0005515 IPI
    sialyltransferase activity GO:0008373 IDA
    beta-galactoside alpha-2,6-sialyltransferase activity GO:0003835 IDA
Biological Process
    protein O-linked glycosylation GO:0006493 TAS
    N-acetylneuraminate metabolic process GO:0006054 IDA
    sialylation GO:0097503 IDA
    post-translational protein modification GO:0043687 TAS
    cellular protein metabolic process GO:0044267 TAS
    humoral immune response GO:0006959 TAS
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 TAS
    O-glycan processing GO:0016266 TAS
    protein N-linked glycosylation via asparagine GO:0018279 TAS
Subcellular Localization
    integral component of Golgi membrane GO:0030173 IEA
    Golgi membrane GO:0000139 TAS
    Golgi cisterna membrane GO:0032580 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified ST6GAL1 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ST6GAL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ST6GAL1 6480
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which ST6GAL1 is involved
PathwayEvidenceSource
N-Glycan antennae elongation TAS Reactome
Sialic acid metabolism TAS Reactome
Termination of O-glycan biosynthesis TAS Reactome





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