Gene description for GABRB3
Gene name gamma-aminobutyric acid (GABA) A receptor, beta 3
Gene symbol GABRB3
Other names/aliases ECA5
Species Homo sapiens
 Database cross references - GABRB3
ExoCarta ExoCarta_2562
Entrez Gene 2562
HGNC 4083
MIM 137192
UniProt P28472  
 GABRB3 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GABRB3
Molecular Function
    GABA-A receptor activity GO:0004890 IMP
    GABA-gated chloride ion channel activity GO:0022851 ISS
    chloride channel activity GO:0005254 IEA
    extracellular ligand-gated ion channel activity GO:0005230 IEA
Biological Process
    transport GO:0006810 TAS
    sensory perception of sound GO:0007605 IEA
    innervation GO:0060384 IEA
    ion transmembrane transport GO:0034220 TAS
    transmembrane transport GO:0055085 TAS
    negative regulation of neuron apoptotic process GO:0043524 IEA
    signal transduction GO:0007165 TAS
    cochlea development GO:0090102 IEA
    palate development GO:0060021 ISS
    chloride transmembrane transport GO:1902476 ISS
    synaptic transmission GO:0007268 TAS
    inner ear receptor cell development GO:0060119 IEA
    cellular response to histamine GO:0071420 IDA
Subcellular Localization
    postsynaptic membrane GO:0045211 IEA
    plasma membrane GO:0005886 TAS
    cell junction GO:0030054 IEA
    GABA-A receptor complex GO:1902711 ISS
    chloride channel complex GO:0034707 IEA
    integral component of plasma membrane GO:0005887 IMP
 Experiment description of studies that identified GABRB3 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GABRB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRKCA 5578
Reconstituted Complex Homo sapiens
2 PRKACA 5566
Reconstituted Complex Homo sapiens
3 AKAP5  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which GABRB3 is involved
PathwayEvidenceSource
GABA A receptor activation TAS Reactome
Ligand-gated ion channel transport TAS Reactome





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