Gene description for Naca
Gene name nascent polypeptide-associated complex alpha polypeptide
Gene symbol Naca
Other names/aliases AL022831
AL024382
Gm1878
mKIAA0363
skNAC
Species Mus musculus
 Database cross references - Naca
ExoCarta ExoCarta_17938
Vesiclepedia VP_17938
Entrez Gene 17938
UniProt P70670   Q60817  
 Naca identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Naca
Molecular Function
    transcription coactivator activity GO:0003713 IDA
    DNA binding GO:0003677 IEA
    TBP-class protein binding GO:0017025 IDA
Biological Process
    regulation of transcription, DNA-templated GO:0006355 IEA
    regulation of skeletal muscle fiber development GO:0048742 IMP
    transport GO:0006810 IEA
    cardiac ventricle development GO:0003231 IMP
    negative regulation of transcription from RNA polymerase II promoter involved in heart development GO:1901227 IMP
    regulation of nucleic acid-templated transcription GO:1903506 IDA
    positive regulation of transcription from RNA polymerase II promoter involved in heart development GO:1901228 IMP
    transcription, DNA-templated GO:0006351 IEA
    skeletal muscle tissue regeneration GO:0043403 IMP
    heart trabecula morphogenesis GO:0061384 IMP
    positive regulation of cell proliferation involved in heart morphogenesis GO:2000138 IMP
    negative regulation of striated muscle cell apoptotic process GO:0010664 IMP
    positive regulation of skeletal muscle tissue growth GO:0048633 IMP
    myoblast migration GO:0051451 IMP
    protein transport GO:0015031 IEA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified Naca in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
EM
EV Biophysical techniques
CD63
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Naca
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Naca is involved
No pathways found





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