Gene description for H2afx
Gene name H2A histone family, member X
Gene symbol H2afx
Other names/aliases AW228881
H2A.X
H2ax
Hist5-2ax
gammaH2ax
Species Mus musculus
 Database cross references - H2afx
ExoCarta ExoCarta_15270
Vesiclepedia VP_15270
Entrez Gene 15270
UniProt P27661  
 H2afx identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for H2afx
Molecular Function
    protein heterodimerization activity GO:0046982 IEA
    protein binding GO:0005515 IPI
    histone binding GO:0042393 ISO
    DNA binding GO:0003677 IEA
    enzyme binding GO:0019899 ISO
    damaged DNA binding GO:0003684 IDA
Biological Process
    cellular response to DNA damage stimulus GO:0006974 ISO
    DNA damage checkpoint GO:0000077 IMP
    meiotic cell cycle GO:0051321 IEA
    DNA recombination GO:0006310 IEA
    DNA repair GO:0006281 IMP
    spermatogenesis GO:0007283 IMP
    cellular response to gamma radiation GO:0071480 ISO
    double-strand break repair via homologous recombination GO:0000724 IMP
    cell cycle GO:0007049 IEA
Subcellular Localization
    nucleosome GO:0000786 IEA
    nucleus GO:0005634 ISO
    nuclear chromatin GO:0000790 IDA
    replication fork GO:0005657 IDA
    male germ cell nucleus GO:0001673 IDA
    condensed nuclear chromosome GO:0000794 IDA
    chromosome, telomeric region GO:0000781 ISO
    chromosome GO:0005694 IDA
    XY body GO:0001741 IDA
    extracellular exosome GO:0070062 ISO
    chromatin GO:0000785 IDA
    site of double-strand break GO:0035861 ISO
 Experiment description of studies that identified H2afx in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for H2afx
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TP53BP1 7158
Co-localization Homo sapiens
2 NBN 4683
Co-localization Homo sapiens
3 ATM 472
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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