Gene description for KATNAL2
Gene name katanin p60 subunit A-like 2
Gene symbol KATNAL2
Other names/aliases -
Species Homo sapiens
 Database cross references - KATNAL2
ExoCarta ExoCarta_83473
Vesiclepedia VP_83473
Entrez Gene 83473
HGNC 25387
MIM 614697
UniProt Q8IYT4  
 KATNAL2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for KATNAL2
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IEA
    microtubule severing ATPase activity GO:0008568 IEA
    isomerase activity GO:0016853 IEA
    ATP hydrolysis activity GO:0016887 IBA
Biological Process
    cytoplasmic microtubule organization GO:0031122 NAS
    microtubule severing GO:0051013 IEA
Subcellular Localization
    spindle pole GO:0000922 IDA
    cytoplasm GO:0005737 IDA
    spindle GO:0005819 IDA
    microtubule GO:0005874 IDA
    katanin complex GO:0008352 ISS
    microtubule cytoskeleton GO:0015630 NAS
 Experiment description of studies that identified KATNAL2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for KATNAL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTN2 88
Two-hybrid Homo sapiens
2 HTATSF1 27336
Co-fractionation Homo sapiens
3 RPLP1 6176
Co-fractionation Homo sapiens
4 DDX1 1653
Co-fractionation Homo sapiens
5 RPA3 6119
Proximity Label-MS Homo sapiens
6 RPA2 6118
Proximity Label-MS Homo sapiens
7 STRIP1 85369
Co-fractionation Homo sapiens
8 RIPK4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which KATNAL2 is involved
No pathways found





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