Gene description for KRT74
Gene name keratin 74, type II
Gene symbol KRT74
Other names/aliases ADWH
HTSS2
HYPT3
K6IRS4
KRT5C
KRT6IRS4
Species Homo sapiens
 Database cross references - KRT74
ExoCarta ExoCarta_121391
Entrez Gene 121391
HGNC 28929
MIM 608248
UniProt Q7RTS7  
 KRT74 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Squamous carcinoma cells 20124223    
 Gene ontology annotations for KRT74
Molecular Function
    structural molecule activity GO:0005198 IEA
    keratin filament binding GO:1990254 IPI
Biological Process
    intermediate filament cytoskeleton organization GO:0045104 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    keratin filament GO:0045095 IEA
 Experiment description of studies that identified KRT74 in exosomes
1
Experiment ID 126
ISEV standards
EV Biophysical techniques
GAPDH
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KRT74
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which KRT74 is involved
No pathways found





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