Gene description for KRT74
Gene name keratin 74, type II
Gene symbol KRT74
Other names/aliases ADWH
HTSS2
HYPT3
K6IRS4
KRT5C
KRT6IRS4
Species Homo sapiens
 Database cross references - KRT74
ExoCarta ExoCarta_121391
Vesiclepedia VP_121391
Entrez Gene 121391
HGNC 28929
MIM 608248
UniProt Q7RTS7  
 KRT74 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Squamous carcinoma cells 20124223    
 Gene ontology annotations for KRT74
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of skin epidermis GO:0030280 IBA
    keratin filament binding GO:1990254 IPI
Biological Process
    keratinization GO:0031424 IBA
    intermediate filament cytoskeleton organization GO:0045104 IDA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    keratin filament GO:0045095 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT74 in exosomes
1
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KRT74
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT6A 3853
Cross-Linking-MS (XL-MS) Homo sapiens
2 KRT35 3886
Two-hybrid Homo sapiens
3 KRT27 342574
Two-hybrid Homo sapiens
4 KRT8 3856
Cross-Linking-MS (XL-MS) Homo sapiens
5 IQCB1  
Affinity Capture-MS Homo sapiens
6 KLF5  
Affinity Capture-MS Homo sapiens
7 KRT19 3880
Cross-Linking-MS (XL-MS) Homo sapiens
Two-hybrid Homo sapiens
8 KRT37 8688
Two-hybrid Homo sapiens
9 KRT15 3866
Two-hybrid Homo sapiens
10 KRT38 8687
Two-hybrid Homo sapiens
11 KRT34  
Two-hybrid Homo sapiens
12 KRT5 3852
Cross-Linking-MS (XL-MS) Homo sapiens
13 LMO4  
Two-hybrid Homo sapiens
14 KRT84 3890
Cross-Linking-MS (XL-MS) Homo sapiens
15 KRT25 147183
Two-hybrid Homo sapiens
16 KRT16 3868
Two-hybrid Homo sapiens
17 ALK 238
Affinity Capture-MS Homo sapiens
18 KRT26 353288
Two-hybrid Homo sapiens
19 RNF208  
Affinity Capture-MS Homo sapiens
20 KRT39  
Two-hybrid Homo sapiens
21 KRT13 3860
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which KRT74 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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