Gene description for APOBEC3B
Gene name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B
Gene symbol APOBEC3B
Other names/aliases A3B
APOBEC1L
ARCD3
ARP4
DJ742C19.2
PHRBNL
bK150C2.2
Species Homo sapiens
 Database cross references - APOBEC3B
ExoCarta ExoCarta_9582
Vesiclepedia VP_9582
Entrez Gene 9582
HGNC 17352
MIM 607110
UniProt Q9UH17  
 APOBEC3B identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Prostate cancer cells 25844599    
 Gene ontology annotations for APOBEC3B
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    cytidine deaminase activity GO:0004126 IBA
    cytidine deaminase activity GO:0004126 IMP
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
Biological Process
    transposable element silencing GO:0010526 IDA
    cytidine to uridine editing GO:0016554 IBA
    clearance of foreign intracellular DNA GO:0044355 IDA
    innate immune response GO:0045087 IEA
    negative regulation of single stranded viral RNA replication via double stranded DNA intermediate GO:0045869 IBA
    defense response to virus GO:0051607 IBA
    defense response to virus GO:0051607 IDA
    DNA cytosine deamination GO:0070383 IBA
Subcellular Localization
    P-body GO:0000932 IBA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified APOBEC3B in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for APOBEC3B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BCLAF1 9774
Affinity Capture-MS Homo sapiens
2 PPP1CB 5500
Affinity Capture-MS Homo sapiens
3 HNRNPH2 3188
Affinity Capture-MS Homo sapiens
4 Fus  
Affinity Capture-MS Mus musculus
5 ZNF346  
Affinity Capture-MS Homo sapiens
6 RPS2 6187
Affinity Capture-MS Homo sapiens
7 LARP4B 23185
Affinity Capture-MS Homo sapiens
8 LIN28A  
Affinity Capture-MS Homo sapiens
9 ECT2 1894
Affinity Capture-MS Homo sapiens
10 APOBEC3D  
Affinity Capture-MS Homo sapiens
11 RBM47 54502
Affinity Capture-MS Homo sapiens
12 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
14 MKI67  
Affinity Capture-MS Homo sapiens
15 Taf15  
Affinity Capture-MS Mus musculus
16 ILF2 3608
Affinity Capture-MS Homo sapiens
17 PPP1CC 5501
Affinity Capture-MS Homo sapiens
18 PRR3  
Affinity Capture-MS Homo sapiens
19 Srsf1 110809
Affinity Capture-MS Mus musculus
20 GSPT2 23708
Affinity Capture-MS Homo sapiens
21 Dctn3  
Affinity Capture-MS Mus musculus
22 SRSF7 6432
Affinity Capture-MS Homo sapiens
23 PABPC5 140886
Affinity Capture-MS Homo sapiens
24 BNIP2  
Two-hybrid Homo sapiens
25 AGR2 10551
Two-hybrid Homo sapiens
26 MATR3 9782
Affinity Capture-MS Homo sapiens
27 Shcbp1 20419
Affinity Capture-MS Mus musculus
28 Snrnp70 20637
Affinity Capture-MS Mus musculus
29 PRC1 9055
Affinity Capture-MS Homo sapiens
30 RBMS2 5939
Affinity Capture-MS Homo sapiens
31 RPL17 6139
Affinity Capture-MS Homo sapiens
32 EED  
Affinity Capture-MS Homo sapiens
33 ABT1 29777
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which APOBEC3B is involved
PathwayEvidenceSource
Formation of the Editosome TAS Reactome
Metabolism of RNA TAS Reactome
mRNA Editing TAS Reactome
mRNA Editing: C to U Conversion TAS Reactome





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