Gene description for SFRP1
Gene name secreted frizzled-related protein 1
Gene symbol SFRP1
Other names/aliases FRP
FRP-1
FRP1
FrzA
SARP2
Species Homo sapiens
 Database cross references - SFRP1
ExoCarta ExoCarta_6422
Vesiclepedia VP_6422
Entrez Gene 6422
HGNC 10776
MIM 604156
UniProt Q8N474  
 SFRP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Saliva 19199708    
 Gene ontology annotations for SFRP1
Molecular Function
    Wnt-activated receptor activity GO:0042813 IBA
    frizzled binding GO:0005109 IPI
    drug binding GO:0008144 IDA
    Wnt-protein binding GO:0017147 IPI
    protein binding GO:0005515 IPI
    G-protein coupled receptor activity GO:0004930 IBA
    identical protein binding GO:0042802 IPI
    cysteine-type endopeptidase activity GO:0004197 IMP
    heparin binding GO:0008201 IDA
Biological Process
    regulation of angiogenesis GO:0045765 ISS
    negative regulation of fibroblast apoptotic process GO:2000270 IDA
    negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification GO:2000054 IDA
    hematopoietic progenitor cell differentiation GO:0002244 IDA
    cellular response to X-ray GO:0071481 IEA
    positive regulation of epithelial cell proliferation GO:0050679 IMP
    cellular response to tumor necrosis factor GO:0071356 IDA
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    positive regulation of canonical Wnt signaling pathway GO:0090263 IDA
    hematopoietic stem cell differentiation GO:0060218 IDA
    negative regulation of canonical Wnt signaling pathway involved in controlling type B pancreatic cell proliferation GO:2000080 IDA
    ureteric bud development GO:0001657 IEA
    negative regulation of fibroblast proliferation GO:0048147 IDA
    Wnt signaling pathway involved in somitogenesis GO:0090244 IEA
    positive regulation of apoptotic process GO:0043065 IMP
    cellular response to prostaglandin E stimulus GO:0071380 IEP
    cellular response to heparin GO:0071504 IDA
    cellular response to estradiol stimulus GO:0071392 IEP
    convergent extension involved in somitogenesis GO:0090246 IEA
    positive regulation of smoothened signaling pathway GO:0045880 IMP
    positive regulation of non-canonical Wnt signaling pathway GO:2000052 IDA
    negative regulation of epithelial cell proliferation GO:0050680 IDA
    negative regulation of epithelial to mesenchymal transition GO:0010719 IDA
    prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis GO:0060527 IEA
    positive regulation of stress fiber assembly GO:0051496 ISS
    cellular response to BMP stimulus GO:0071773 IEP
    negative regulation of cell proliferation GO:0008285 IDA
    cellular response to interleukin-1 GO:0071347 IEP
    cellular response to transforming growth factor beta stimulus GO:0071560 IEP
    dorsal/ventral axis specification GO:0009950 IDA
    response to drug GO:0042493 IDA
    positive regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902043 IDA
    negative regulation of gene expression GO:0010629 IDA
    bone trabecula formation GO:0060346 IEA
    negative regulation of apoptotic process GO:0043066 IMP
    positive regulation of fat cell differentiation GO:0045600 IDA
    positive regulation of fibroblast apoptotic process GO:2000271 IDA
    cellular response to estrogen stimulus GO:0071391 IDA
    negative regulation of planar cell polarity pathway involved in axis elongation GO:2000041 IEA
    positive regulation of focal adhesion assembly GO:0051894 ISS
    osteoblast differentiation GO:0001649 IEP
    negative regulation of insulin secretion GO:0046676 IDA
    cellular response to hypoxia GO:0071456 IEP
    regulation of cell cycle process GO:0010564 IMP
    canonical Wnt signaling pathway GO:0060070 IDA
    cellular response to fibroblast growth factor stimulus GO:0044344 IDA
    positive regulation of GTPase activity GO:0043547 ISS
    negative regulation of osteoblast differentiation GO:0045668 IEA
    negative regulation of Wnt signaling pathway GO:0030178 IMP
    menstrual cycle phase GO:0022601 IEP
    negative regulation of JUN kinase activity GO:0043508 IEA
    cellular response to vitamin D GO:0071305 IEP
    negative regulation of cell migration GO:0030336 IDA
    negative regulation of B cell differentiation GO:0045578 IMP
    response to organic cyclic compound GO:0014070 IDA
    digestive tract morphogenesis GO:0048546 IEA
    negative regulation of cell growth GO:0030308 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    regulation of branching involved in prostate gland morphogenesis GO:0060687 IEA
    negative regulation of androgen receptor signaling pathway GO:0060766 IDA
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    male gonad development GO:0008584 IEA
    female gonad development GO:0008585 IEA
    negative regulation of BMP signaling pathway GO:0030514 IEA
    negative regulation of osteoblast proliferation GO:0033689 IMP
    positive regulation of cell-matrix adhesion GO:0001954 ISS
    planar cell polarity pathway involved in neural tube closure GO:0090179 IEA
    neural crest cell fate commitment GO:0014034 IEA
    cellular response to starvation GO:0009267 IEP
    non-canonical Wnt signaling pathway GO:0035567 IBA
    stromal-epithelial cell signaling involved in prostate gland development GO:0044345 IEA
    positive regulation of Wnt signaling pathway GO:0030177 IDA
    negative regulation of ossification GO:0030279 IMP
    positive regulation of cell proliferation GO:0008284 IDA
    somatic stem cell maintenance GO:0035019 IEA
    negative regulation of osteoclast differentiation GO:0045671 IEA
    negative regulation of peptidyl-tyrosine phosphorylation GO:0050732 IDA
    negative regulation of bone remodeling GO:0046851 IMP
    proteolysis GO:0006508 IMP
    positive regulation of cell growth GO:0030307 IDA
    cellular response to growth factor stimulus GO:0071363 IDA
Subcellular Localization
    extracellular space GO:0005615 IDA
    cytosol GO:0005829 IDA
    extracellular region GO:0005576 TAS
    plasma membrane GO:0005886 ISS
    extracellular matrix GO:0031012 IDA
    cell surface GO:0009986 IDA
    extracellular exosome GO:0070062 IDA
    integral component of membrane GO:0016021 IBA
    proteinaceous extracellular matrix GO:0005578 IDA
    intracellular GO:0005622 IDA
 Experiment description of studies that identified SFRP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 497
MISEV standards
✘ EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 258
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1|CD81
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 259
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 260
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|FLOT1|CD81
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
13
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 66
MISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix|CD63|CD81
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for SFRP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FZD6 8323
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 WNT2  
Affinity Capture-Western Homo sapiens
3 WNT4  
Invivo Homo sapiens
4 WNT1  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SFRP1 is involved
PathwayEvidenceSource
negative regulation of TCF-dependent signaling by WNT ligand antagonists TAS Reactome





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