Gene description for Ppp1r12a
Gene name protein phosphatase 1, regulatory (inhibitor) subunit 12A
Gene symbol Ppp1r12a
Other names/aliases 1200015F06Rik
5730577I22Rik
AA792106
AV099298
D10Ertd625e
Mypt1
Species Mus musculus
 Database cross references - Ppp1r12a
ExoCarta ExoCarta_17931
Entrez Gene 17931
UniProt Q9DBR7  
 Ppp1r12a identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ppp1r12a
Molecular Function
    phosphatase regulator activity GO:0019208 ISO
    phosphoprotein phosphatase activity GO:0004721 ISO
    enzyme inhibitor activity GO:0004857 ISO
    protein kinase binding GO:0019901 ISO
    14-3-3 protein binding GO:0071889 ISO
    protein binding GO:0005515 IPI
Biological Process
    mitotic nuclear division GO:0007067 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    protein dephosphorylation GO:0006470 ISO
    regulation of myosin-light-chain-phosphatase activity GO:0035507 ISO
    signal transduction GO:0007165 IEA
    negative regulation of catalytic activity GO:0043086 ISO
    centrosome organization GO:0051297 ISO
    regulation of nucleocytoplasmic transport GO:0046822 IMP
    regulation of cell adhesion GO:0030155 ISO
Subcellular Localization
    PTW/PP1 phosphatase complex GO:0072357 ISO
    kinetochore GO:0000776 ISO
    actin cytoskeleton GO:0015629 ISO
    contractile fiber GO:0043292 ISO
    focal adhesion GO:0005925 ISO
    centrosome GO:0005813 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Ppp1r12a in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ppp1r12a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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