Gene description for Ezr
Gene name ezrin
Gene symbol Ezr
Other names/aliases AW146364
R75297
Vil2
p81
Species Mus musculus
 Database cross references - Ezr
ExoCarta ExoCarta_22350
Vesiclepedia VP_22350
Entrez Gene 22350
UniProt P26040  
 Ezr identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Faeces 33431899    
Faeces 33431899    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Microglia 16081791    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ezr
Molecular Function
    protein domain specific binding GO:0019904 ISO
    protein complex binding GO:0032403 ISO
    actin binding GO:0003779 IEA
    cytoskeletal protein binding GO:0008092 IEA
    protein binding GO:0005515 IPI
    cell adhesion molecule binding GO:0050839 ISO
    poly(A) RNA binding GO:0044822 ISO
    actin filament binding GO:0051015 ISO
Biological Process
    actin filament bundle assembly GO:0051017 ISO
    regulation of microvillus length GO:0032532 IGI
    positive regulation of establishment of protein localization to plasma membrane GO:0090004 IGI
    microvillus assembly GO:0030033 IGI
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of early endosome to late endosome transport GO:2000643 ISO
    positive regulation of multicellular organism growth GO:0040018 IGI
    intestinal D-glucose absorption GO:0001951 IGI
    filopodium assembly GO:0046847 ISO
    terminal web assembly GO:1902896 IGI
    sphingosine-1-phosphate signaling pathway GO:0003376 ISO
    receptor internalization GO:0031623 IMP
    regulation of organelle assembly GO:1902115 ISO
    actin cytoskeleton reorganization GO:0031532 ISO
    positive regulation of protein secretion GO:0050714 ISO
    establishment or maintenance of apical/basal cell polarity GO:0035088 IMP
    positive regulation of protein localization to early endosome GO:1902966 ISO
    establishment of endothelial barrier GO:0061028 ISO
    regulation of cell shape GO:0008360 IEA
    phosphatidylinositol-mediated signaling GO:0048015 ISO
    positive regulation of cellular protein catabolic process GO:1903364 ISO
Subcellular Localization
    focal adhesion GO:0005925 ISO
    T-tubule GO:0030315 ISO
    uropod GO:0001931 IDA
    plasma membrane GO:0005886 ISO
    extracellular exosome GO:0070062 ISO
    Schwann cell microvillus GO:0097454 ISO
    microspike GO:0044393 ISO
    cell tip GO:0051286 ISO
    ciliary basal body GO:0036064 IDA
    extracellular space GO:0005615 ISO
    brush border GO:0005903 IDA
    ruffle GO:0001726 ISO
    cell projection GO:0042995 IEA
    cell periphery GO:0071944 ISO
    actin cytoskeleton GO:0015629 ISO
    myelin sheath GO:0043209 IDA
    actin filament GO:0005884 ISO
    microvillus GO:0005902 ISO
    apical part of cell GO:0045177 ISO
    basolateral plasma membrane GO:0016323 ISO
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
    cytosol GO:0005829 ISO
    endosome GO:0005768 ISO
    membrane raft GO:0045121 ISO
    cytoplasmic side of apical plasma membrane GO:0098592 ISO
    nucleolus GO:0005730 ISO
    intracellular GO:0005622 IDA
    astrocyte projection GO:0097449 ISO
    apical plasma membrane GO:0016324 ISO
    membrane GO:0016020 ISO
    microvillus membrane GO:0031528 ISO
    extrinsic component of membrane GO:0019898 ISO
    cell body GO:0044297 ISO
    filopodium GO:0030175 ISO
    vesicle GO:0031982 ISO
 Experiment description of studies that identified Ezr in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 303
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd63|Cd81|Cd9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG.
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
3
Experiment ID 304
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd63|Cd81|Cd9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG.
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
4
Experiment ID 214
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 215
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 216
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
8
Experiment ID 14
MISEV standards
✔
EM
EV Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
EV Enriched markers
✔
DNM
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
9
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ezr
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tsc1  
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which Ezr is involved
PathwayEvidenceSource
Netrin-1 signaling IEA Reactome
Recycling pathway of L1 IEA Reactome





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