Gene description for Ezr
Gene name ezrin
Gene symbol Ezr
Other names/aliases AW146364
R75297
Vil2
p81
Species Mus musculus
 Database cross references - Ezr
ExoCarta ExoCarta_22350
Vesiclepedia VP_22350
Entrez Gene 22350
UniProt P26040  
 Ezr identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Faeces 33431899    
Faeces 33431899    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Microglia 16081791    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ezr
Molecular Function
    actin binding GO:0003779 IBA
    actin binding GO:0003779 ISO
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IEA
    microtubule binding GO:0008017 ISO
    protein domain specific binding GO:0019904 ISO
    protein kinase A catalytic subunit binding GO:0034236 IEA
    protein kinase A catalytic subunit binding GO:0034236 ISO
    protein kinase A regulatory subunit binding GO:0034237 IEA
    protein kinase A regulatory subunit binding GO:0034237 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    S100 protein binding GO:0044548 IEA
    S100 protein binding GO:0044548 ISO
    protein-containing complex binding GO:0044877 ISO
    cell adhesion molecule binding GO:0050839 IBA
    cell adhesion molecule binding GO:0050839 ISO
    cell adhesion molecule binding GO:0050839 ISS
    actin filament binding GO:0051015 ISO
    actin filament binding GO:0051015 ISS
    protein kinase A binding GO:0051018 ISO
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
    disordered domain specific binding GO:0097718 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    intestinal D-glucose absorption GO:0001951 IGI
    sphingosine-1-phosphate receptor signaling pathway GO:0003376 IEA
    sphingosine-1-phosphate receptor signaling pathway GO:0003376 ISO
    leukocyte cell-cell adhesion GO:0007159 IEA
    regulation of cell shape GO:0008360 IBA
    regulation of cell shape GO:0008360 IEA
    regulation of cell shape GO:0008360 ISO
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    protein kinase A signaling GO:0010737 IEA
    protein kinase A signaling GO:0010737 ISO
    membrane to membrane docking GO:0022614 IEA
    microvillus assembly GO:0030033 IGI
    microvillus assembly GO:0030033 ISO
    actin cytoskeleton organization GO:0030036 ISO
    astral microtubule organization GO:0030953 IEA
    astral microtubule organization GO:0030953 ISO
    protein-containing complex localization GO:0031503 IEA
    protein-containing complex localization GO:0031503 ISO
    receptor internalization GO:0031623 IMP
    regulation of microvillus length GO:0032532 IGI
    negative regulation of interleukin-2 production GO:0032703 IEA
    negative regulation of interleukin-2 production GO:0032703 ISO
    establishment or maintenance of apical/basal cell polarity GO:0035088 IMP
    positive regulation of multicellular organism growth GO:0040018 IGI
    cortical microtubule organization GO:0043622 IEA
    cortical microtubule organization GO:0043622 ISO
    positive regulation of protein catabolic process GO:0045732 IEA
    positive regulation of protein catabolic process GO:0045732 ISO
    filopodium assembly GO:0046847 IEA
    filopodium assembly GO:0046847 ISO
    negative regulation of T cell receptor signaling pathway GO:0050860 IEA
    negative regulation of T cell receptor signaling pathway GO:0050860 ISO
    actin filament bundle assembly GO:0051017 ISO
    actin filament bundle assembly GO:0051017 ISS
    establishment of centrosome localization GO:0051660 IEA
    establishment of centrosome localization GO:0051660 ISO
    establishment of endothelial barrier GO:0061028 IEA
    establishment of endothelial barrier GO:0061028 ISO
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    cellular response to cAMP GO:0071320 IEA
    cellular response to cAMP GO:0071320 ISO
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    protein localization to cell cortex GO:0072697 IEA
    protein localization to cell cortex GO:0072697 ISO
    postsynaptic actin cytoskeleton organization GO:0098974 IDA
    postsynaptic actin cytoskeleton organization GO:0098974 IMP
    regulation of organelle assembly GO:1902115 IBA
    regulation of organelle assembly GO:1902115 IEA
    regulation of organelle assembly GO:1902115 ISO
    terminal web assembly GO:1902896 IGI
    positive regulation of protein localization to early endosome GO:1902966 IBA
    positive regulation of protein localization to early endosome GO:1902966 IEA
    positive regulation of protein localization to early endosome GO:1902966 ISO
    positive regulation of protein localization to plasma membrane GO:1903078 IGI
    negative regulation of p38MAPK cascade GO:1903753 IEA
    negative regulation of p38MAPK cascade GO:1903753 ISO
    positive regulation of early endosome to late endosome transport GO:2000643 IBA
    positive regulation of early endosome to late endosome transport GO:2000643 IEA
    positive regulation of early endosome to late endosome transport GO:2000643 ISO
Subcellular Localization
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    ruffle GO:0001726 ISO
    immunological synapse GO:0001772 IEA
    immunological synapse GO:0001772 ISO
    immunological synapse GO:0001772 ISO
    uropod GO:0001931 IDA
    intracellular anatomical structure GO:0005622 IDA
    cytoplasm GO:0005737 ISO
    endosome GO:0005768 IEA
    endosome GO:0005768 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    actin filament GO:0005884 ISO
    actin filament GO:0005884 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    microvillus GO:0005902 IBA
    microvillus GO:0005902 IDA
    microvillus GO:0005902 ISO
    brush border GO:0005903 IDA
    adherens junction GO:0005912 IBA
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    cell cortex GO:0005938 IEA
    actin cytoskeleton GO:0015629 ISO
    actin cytoskeleton GO:0015629 ISS
    basolateral plasma membrane GO:0016323 ISO
    basolateral plasma membrane GO:0016323 ISS
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    filopodium GO:0030175 IBA
    filopodium GO:0030175 IEA
    filopodium GO:0030175 ISO
    T-tubule GO:0030315 ISO
    microvillus membrane GO:0031528 IEA
    microvillus membrane GO:0031528 ISO
    ruffle membrane GO:0032587 IEA
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    ciliary basal body GO:0036064 IDA
    cell projection GO:0042995 ISO
    myelin sheath GO:0043209 HDA
    cell body GO:0044297 ISO
    microspike GO:0044393 ISO
    plasma membrane raft GO:0044853 IEA
    plasma membrane raft GO:0044853 ISO
    apical part of cell GO:0045177 IBA
    apical part of cell GO:0045177 IDA
    apical part of cell GO:0045177 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    cell tip GO:0051286 ISO
    cell periphery GO:0071944 ISO
    astrocyte projection GO:0097449 ISO
    Schwann cell microvillus GO:0097454 ISO
 Experiment description of studies that identified Ezr in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
3
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
4
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
8
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
9
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ezr
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tbc1d10a  
Co-fractionation Mus musculus
2 Slc12a2 20496
Co-fractionation Mus musculus
3 Ubc  
Reconstituted Complex Mus musculus
4 TRAPPC9 83696
Affinity Capture-MS Homo sapiens
5 Pde4dip  
Affinity Capture-MS Mus musculus
6 TBC1D7  
Affinity Capture-MS Homo sapiens
7 Tsc1  
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
8 Slc9a1  
Co-fractionation Mus musculus
9 Bid  
Co-fractionation Mus musculus
10 Atg16l1  
Affinity Capture-MS Mus musculus
11 Ptk2  
Co-fractionation Mus musculus
12 Tmem173  
Proximity Label-MS Mus musculus
13 RDX 5962
Affinity Capture-MS Homo sapiens
14 IBTK 25998
Affinity Capture-MS Homo sapiens
15 Rhoa 11848
Co-fractionation Mus musculus
16 Ube2r2  
Co-fractionation Mus musculus
17 Nf2  
Co-fractionation Mus musculus
18 SRRM2 23524
Affinity Capture-MS Homo sapiens
19 Eed  
Affinity Capture-MS Mus musculus
20 MSN 4478
Affinity Capture-MS Homo sapiens
21 Traf3  
Proximity Label-MS Mus musculus
22 Slc9a3r1 26941
Co-fractionation Mus musculus
23 Fancd2  
Affinity Capture-MS Mus musculus
24 Snx27 76742
Co-fractionation Mus musculus
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