Gene description for Slc9a3r1
Gene name solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
Gene symbol Slc9a3r1
Other names/aliases EBP-50
NHE-RF
NHERF-1
NHERF1
Species Mus musculus
 Database cross references - Slc9a3r1
ExoCarta ExoCarta_26941
Vesiclepedia VP_26941
Entrez Gene 26941
UniProt P70441  
 Slc9a3r1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Slc9a3r1
Molecular Function
    signaling receptor binding GO:0005102 IBA
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IEA
    beta-catenin binding GO:0008013 ISO
    gamma-aminobutyric acid transmembrane transporter activity GO:0015185 IEA
    gamma-aminobutyric acid transmembrane transporter activity GO:0015185 ISO
    chloride channel regulator activity GO:0017081 ISO
    chloride channel regulator activity GO:0017081 ISS
    phosphatase binding GO:0019902 IPI
    phosphatase binding GO:0019902 ISO
    protein domain specific binding GO:0019904 ISO
    PDZ domain binding GO:0030165 IEA
    PDZ domain binding GO:0030165 ISO
    beta-2 adrenergic receptor binding GO:0031698 IEA
    beta-2 adrenergic receptor binding GO:0031698 ISO
    type 2 metabotropic glutamate receptor binding GO:0031799 IEA
    type 2 metabotropic glutamate receptor binding GO:0031799 ISO
    type 3 metabotropic glutamate receptor binding GO:0031800 IEA
    type 3 metabotropic glutamate receptor binding GO:0031800 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein-membrane adaptor activity GO:0043495 IBA
    transmembrane transporter binding GO:0044325 IEA
    transmembrane transporter binding GO:0044325 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    myosin II binding GO:0045159 IEA
    myosin II binding GO:0045159 ISO
    dopamine receptor binding GO:0050780 IDA
    molecular adaptor activity GO:0060090 IEA
    molecular adaptor activity GO:0060090 ISO
    growth factor receptor binding GO:0070851 IEA
    growth factor receptor binding GO:0070851 ISO
    channel activator activity GO:0099103 IEA
    channel activator activity GO:0099103 ISO
Biological Process
    renal sodium ion transport GO:0003096 IMP
    sodium ion transport GO:0006814 IMP
    plasma membrane organization GO:0007009 IMP
    nuclear migration GO:0007097 IEA
    nuclear migration GO:0007097 ISO
    adenylate cyclase-activating dopamine receptor signaling pathway GO:0007191 IMP
    sensory perception of sound GO:0007605 IMP
    protein localization GO:0008104 IMP
    negative regulation of cell population proliferation GO:0008285 IEA
    negative regulation of cell population proliferation GO:0008285 ISO
    regulation of cell shape GO:0008360 IEA
    regulation of cell shape GO:0008360 ISO
    regulation of cell size GO:0008361 IEA
    regulation of cell size GO:0008361 ISO
    negative regulation of platelet-derived growth factor receptor signaling pathway GO:0010642 IMP
    fibroblast migration GO:0010761 IGI
    negative regulation of fibroblast migration GO:0010764 IGI
    negative regulation of sodium ion transport GO:0010766 IMP
    Wnt signaling pathway GO:0016055 IEA
    gland morphogenesis GO:0022612 IEA
    gland morphogenesis GO:0022612 ISO
    microvillus assembly GO:0030033 IEA
    microvillus assembly GO:0030033 ISO
    actin cytoskeleton organization GO:0030036 IMP
    intracellular phosphate ion homeostasis GO:0030643 IMP
    negative regulation of sodium:proton antiporter activity GO:0032416 IMP
    bile acid secretion GO:0032782 IMP
    glutathione transport GO:0034635 IMP
    positive regulation of monoatomic ion transmembrane transport GO:0034767 IEA
    positive regulation of monoatomic ion transmembrane transport GO:0034767 ISO
    cilium organization GO:0044782 IMP
    establishment of epithelial cell apical/basal polarity GO:0045198 IEA
    establishment of epithelial cell apical/basal polarity GO:0045198 ISO
    maintenance of epithelial cell apical/basal polarity GO:0045199 IMP
    maintenance of epithelial cell apical/basal polarity GO:0045199 ISO
    regulation of protein kinase activity GO:0045859 IMP
    negative regulation of mitotic cell cycle GO:0045930 IEA
    negative regulation of mitotic cell cycle GO:0045930 ISO
    establishment of localization in cell GO:0051649 IMP
    establishment of localization in cell GO:0051649 IMP
    establishment of Golgi localization GO:0051683 IEA
    establishment of Golgi localization GO:0051683 ISO
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IGI
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 IMP
    negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051898 ISO
    gamma-aminobutyric acid import GO:0051939 IEA
    gamma-aminobutyric acid import GO:0051939 ISO
    auditory receptor cell stereocilium organization GO:0060088 IMP
    phospholipase C-activating dopamine receptor signaling pathway GO:0060158 IMP
    renal absorption GO:0070293 IMP
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    negative regulation of canonical Wnt signaling pathway GO:0090090 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 ISO
    cerebrospinal fluid circulation GO:0090660 IDA
    cerebrospinal fluid circulation GO:0090660 IMP
    renal phosphate ion absorption GO:0097291 ISO
    renal phosphate ion absorption GO:0097291 ISS
    import across plasma membrane GO:0098739 IEA
    import across plasma membrane GO:0098739 ISO
    regulation of renal phosphate excretion GO:1903402 IMP
    negative regulation of cell motility GO:2000146 IMP
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 IEA
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISO
Subcellular Localization
    ruffle GO:0001726 IEA
    extracellular region GO:0005576 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    microvillus GO:0005902 ISO
    microvillus GO:0005902 ISS
    endomembrane system GO:0012505 IEA
    membrane GO:0016020 IDA
    membrane GO:0016020 ISO
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    filopodium GO:0030175 IEA
    brush border membrane GO:0031526 IDA
    microvillus membrane GO:0031528 ISO
    microvillus membrane GO:0031528 ISS
    stereocilium GO:0032420 IDA
    stereocilium tip GO:0032426 IDA
    apical part of cell GO:0045177 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    cell periphery GO:0071944 ISO
    sperm midpiece GO:0097225 IDA
    plasma membrane protein complex GO:0098797 IEA
    plasma membrane protein complex GO:0098797 ISO
 Experiment description of studies that identified Slc9a3r1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Slc9a3r1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tbc1d10a  
Co-fractionation Mus musculus
2 Pik3ca  
Co-fractionation Mus musculus
3 Nf2  
Co-fractionation Mus musculus
4 Slc34a1  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
5 Ezr 22350
Co-fractionation Mus musculus
6 Dak  
Co-fractionation Mus musculus
7 Rlbp1  
Co-fractionation Mus musculus
8 Plcb3 18797
Co-fractionation Mus musculus
9 Cftr  
Affinity Capture-Western Mus musculus
10 SLC9A3 6550
Two-hybrid Homo sapiens
11 Gna11 14672
Co-fractionation Mus musculus
12 Pten  
Co-fractionation Mus musculus
13 Snx27 76742
Co-fractionation Mus musculus
14 Tgm2  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Slc9a3r1 is involved
No pathways found





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