Gene description for Arhgdib
Gene name Rho, GDP dissociation inhibitor (GDI) beta
Gene symbol Arhgdib
Other names/aliases D4
Gdid4
Ly-GDI
Species Mus musculus
 Database cross references - Arhgdib
ExoCarta ExoCarta_11857
Vesiclepedia VP_11857
Entrez Gene 11857
UniProt Q61599  
 Arhgdib identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Arhgdib
Molecular Function
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    Rho GDP-dissociation inhibitor activity GO:0005094 IBA
    Rho GDP-dissociation inhibitor activity GO:0005094 ISO
    Rho GDP-dissociation inhibitor activity GO:0005094 ISS
    GTPase activator activity GO:0005096 IEA
    small GTPase binding GO:0031267 ISO
    small GTPase binding GO:0031267 ISS
Biological Process
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 IEA
    regulation of Rho protein signal transduction GO:0035023 ISO
    regulation of Rho protein signal transduction GO:0035023 ISS
    negative regulation of trophoblast cell migration GO:1901164 IEA
    negative regulation of trophoblast cell migration GO:1901164 ISO
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    membrane GO:0016020 IBA
 Experiment description of studies that identified Arhgdib in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Arhgdib
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sstr3  
Proximity Label-MS Mus musculus
2 Rhoa 11848
Co-fractionation Mus musculus
3 RNF128  
Affinity Capture-Western Homo sapiens
4 Tmem173  
Proximity Label-MS Mus musculus
5 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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