Gene description for Eif3d
Gene name eukaryotic translation initiation factor 3, subunit D
Gene symbol Eif3d
Other names/aliases 66/67kDa
AA407891
Eif3s7
eIF3p66
Species Mus musculus
 Database cross references - Eif3d
ExoCarta ExoCarta_55944
Vesiclepedia VP_55944
Entrez Gene 55944
UniProt O70194  
 Eif3d identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif3d
Molecular Function
    RNA binding GO:0003723 ISO
    translation initiation factor activity GO:0003743 IDA
    translation initiation factor activity GO:0003743 ISO
    mRNA cap binding GO:0098808 ISO
    mRNA cap binding GO:0098808 ISS
Biological Process
    formation of cytoplasmic translation initiation complex GO:0001732 IDA
    cap-dependent translational initiation GO:0002191 ISO
    cap-dependent translational initiation GO:0002191 ISS
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 ISO
    positive regulation of translation GO:0045727 IEA
    positive regulation of translation GO:0045727 ISO
    IRES-dependent viral translational initiation GO:0075522 IEA
    IRES-dependent viral translational initiation GO:0075522 ISO
    viral translational termination-reinitiation GO:0075525 IEA
    viral translational termination-reinitiation GO:0075525 ISO
    positive regulation of mRNA binding GO:1902416 ISO
Subcellular Localization
    eukaryotic translation initiation factor 3 complex GO:0005852 IBA
    eukaryotic translation initiation factor 3 complex GO:0005852 IDA
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    eukaryotic translation initiation factor 3 complex, eIF3m GO:0071541 IDA
 Experiment description of studies that identified Eif3d in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif3d
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rpl35 66489
Co-fractionation Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Flad1  
Co-fractionation Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Fzr1  
Affinity Capture-MS Mus musculus
6 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
7 Eed  
Affinity Capture-MS Mus musculus
8 Atg16l1  
Affinity Capture-MS Mus musculus
9 Chaf1a  
Affinity Capture-MS Mus musculus
10 ZRSR2  
Two-hybrid Homo sapiens
11 EXOSC8  
Two-hybrid Homo sapiens
12 Dohh  
Co-fractionation Mus musculus
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