Gene description for MICAL2
Gene name microtubule associated monooxygenase, calponin and LIM domain containing 2
Gene symbol MICAL2
Other names/aliases MICAL-2
MICAL2PV1
MICAL2PV2
Species Homo sapiens
 Database cross references - MICAL2
ExoCarta ExoCarta_9645
Vesiclepedia VP_9645
Entrez Gene 9645
HGNC 24693
MIM 608881
UniProt O94851  
 MICAL2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MICAL2
Molecular Function
    actin binding GO:0003779 IDA
    actin binding GO:0003779 ISS
    monooxygenase activity GO:0004497 IDA
    protein binding GO:0005515 IPI
    NAD(P)H oxidase H2O2-forming activity GO:0016174 IEA
    oxidoreductase activity GO:0016491 IDA
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen GO:0016709 ISS
    metal ion binding GO:0046872 IEA
    mitogen-activated protein kinase binding GO:0051019 ISS
    FAD binding GO:0071949 ISS
    F-actin monooxygenase activity GO:0120501 IEA
Biological Process
    heart looping GO:0001947 ISS
    cytoskeleton organization GO:0007010 IDA
    heart development GO:0007507 ISS
    sulfur oxidation GO:0019417 ISS
    actin filament depolymerization GO:0030042 IDA
    actin filament depolymerization GO:0030042 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 ISS
    actin filament GO:0005884 IDA
 Experiment description of studies that identified MICAL2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MICAL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 TRIM54  
Two-hybrid Homo sapiens
3 COPA 1314
Affinity Capture-MS Homo sapiens
4 MAP2K3 5606
Affinity Capture-MS Homo sapiens
5 BCAP31 10134
Affinity Capture-MS Homo sapiens
6 PGRMC1 10857
Affinity Capture-MS Homo sapiens
7 ARHGAP15 55843
Affinity Capture-MS Homo sapiens
8 TTYH1  
Affinity Capture-MS Homo sapiens
9 KIF23 9493
Affinity Capture-MS Homo sapiens
10 KIF14 9928
Affinity Capture-MS Homo sapiens
11 COPE 11316
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 COPB2 9276
Affinity Capture-MS Homo sapiens
14 LINC00839  
Protein-RNA Homo sapiens
View the network image/svg+xml
 Pathways in which MICAL2 is involved
No pathways found





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