Gene description for TRIB1
Gene name tribbles pseudokinase 1
Gene symbol TRIB1
Other names/aliases C8FW
GIG-2
GIG2
SKIP1
TRB-1
TRB1
Species Homo sapiens
 Database cross references - TRIB1
ExoCarta ExoCarta_10221
Vesiclepedia VP_10221
Entrez Gene 10221
HGNC 16891
MIM 609461
UniProt Q96RU8  
 TRIB1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for TRIB1
Molecular Function
    protein kinase activity GO:0004672 TAS
    protein kinase inhibitor activity GO:0004860 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 TAS
    mitogen-activated protein kinase kinase binding GO:0031434 IBA
    mitogen-activated protein kinase kinase binding GO:0031434 IDA
    ubiquitin protein ligase binding GO:0031625 ISS
    ubiquitin-protein transferase regulator activity GO:0055106 ISS
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
Biological Process
    protein phosphorylation GO:0006468 TAS
    negative regulation of protein kinase activity GO:0006469 IMP
    JNK cascade GO:0007254 IMP
    negative regulation of smooth muscle cell migration GO:0014912 IMP
    negative regulation of lipopolysaccharide-mediated signaling pathway GO:0031665 IMP
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IBA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISS
    response to lipopolysaccharide GO:0032496 IMP
    response to lipopolysaccharide GO:0032496 ISS
    regulation of MAP kinase activity GO:0043405 IDA
    negative regulation of DNA-binding transcription factor activity GO:0043433 IMP
    negative regulation of DNA-binding transcription factor activity GO:0043433 ISS
    positive regulation of eosinophil differentiation GO:0045645 IEA
    positive regulation of macrophage differentiation GO:0045651 IEA
    negative regulation of neutrophil differentiation GO:0045659 IEA
    negative regulation of smooth muscle cell proliferation GO:0048662 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified TRIB1 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TRIB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 INHBE 83729
Affinity Capture-MS Homo sapiens
2 TSPY2  
Affinity Capture-MS Homo sapiens
3 RASSF8 11228
Affinity Capture-MS Homo sapiens
4 MLXIPL  
Affinity Capture-MS Homo sapiens
5 ATF6  
Affinity Capture-MS Homo sapiens
6 PCDH1 5097
Affinity Capture-MS Homo sapiens
7 RFWD2  
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 FOSL1  
Affinity Capture-MS Homo sapiens
9 STK17B 9262
Affinity Capture-MS Homo sapiens
10 LLGL1 3996
Affinity Capture-MS Homo sapiens
11 ISCA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 UBE2E1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
14 MORC3 23515
Affinity Capture-MS Homo sapiens
15 TRIB2 28951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 FOSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SLAIN2  
Affinity Capture-MS Homo sapiens
18 PECR 55825
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 FOS 2353
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 BECN1 8678
Affinity Capture-MS Homo sapiens
21 MYC  
Dosage Lethality Homo sapiens
22 STK40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 KLC2 64837
Two-hybrid Homo sapiens
24 HNRNPA1L2 144983
Affinity Capture-MS Homo sapiens
25 ALOX12 239
Two-hybrid Homo sapiens
26 JUNB  
Affinity Capture-MS Homo sapiens
27 MALT1 10892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 FOSB  
Affinity Capture-MS Homo sapiens
29 DET1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 HSPB8 26353
Affinity Capture-MS Homo sapiens
31 UBXN6 80700
Affinity Capture-MS Homo sapiens
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