Gene description for ADH1B
Gene name alcohol dehydrogenase 1B (class I), beta polypeptide
Gene symbol ADH1B
Other names/aliases ADH2
HEL-S-117
Species Homo sapiens
 Database cross references - ADH1B
ExoCarta ExoCarta_125
Vesiclepedia VP_125
Entrez Gene 125
HGNC 250
MIM 103720
UniProt P00325  
 ADH1B identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ADH1B
Molecular Function
    alcohol dehydrogenase (NAD+) activity GO:0004022 IBA
    alcohol dehydrogenase (NAD+) activity GO:0004022 IDA
    all-trans-retinol dehydrogenase (NAD+) activity GO:0004745 IBA
    all-trans-retinol dehydrogenase (NAD+) activity GO:0004745 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IDA
Biological Process
    retinoid metabolic process GO:0001523 IDA
    retinol metabolic process GO:0042572 IBA
    retinoic acid metabolic process GO:0042573 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified ADH1B in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ADH1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADH1C 126
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 YWHAE 7531
Affinity Capture-MS Homo sapiens
3 PDE4DIP 9659
Two-hybrid Homo sapiens
4 PML 5371
Two-hybrid Homo sapiens
5 GNB2L1 10399
Two-hybrid Homo sapiens
6 ADH1A 124
Affinity Capture-MS Homo sapiens
7 SERPINA3 12
Two-hybrid Homo sapiens
8 VCP 7415
Affinity Capture-MS Homo sapiens
9 IRAK3 11213
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ADH1B is involved
PathwayEvidenceSource
Biological oxidations TAS Reactome
Ethanol oxidation TAS Reactome
Metabolism TAS Reactome
Phase I - Functionalization of compounds TAS Reactome





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