Gene description for POLN
Gene name polymerase (DNA directed) nu
Gene symbol POLN
Other names/aliases POL4P
Species Homo sapiens
 Database cross references - POLN
ExoCarta ExoCarta_353497
Vesiclepedia VP_353497
Entrez Gene 353497
HGNC 18870
MIM 610887
UniProt Q7Z5Q5  
 POLN identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for POLN
Molecular Function
    DNA binding GO:0003677 IEA
    DNA-directed DNA polymerase activity GO:0003887 IBA
    DNA-directed DNA polymerase activity GO:0003887 IDA
    DNA-directed DNA polymerase activity GO:0003887 TAS
    protein binding GO:0005515 IPI
    cyclin binding GO:0030332 IPI
Biological Process
    double-strand break repair via homologous recombination GO:0000724 IMP
    DNA-templated DNA replication GO:0006261 IEA
    double-strand break repair GO:0006302 IBA
    translesion synthesis GO:0019985 IDA
    translesion synthesis GO:0019985 IMP
    interstrand cross-link repair GO:0036297 IMP
    interstrand cross-link repair GO:0036297 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified POLN in sEVs
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for POLN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLDIP2 26073
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
2 ATR  
Biochemical Activity Homo sapiens
3 PCNA 5111
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
4 FANCA  
Affinity Capture-Western Homo sapiens
5 FANCG 2189
Affinity Capture-Western Homo sapiens
6 FANCI 55215
Affinity Capture-Western Homo sapiens
7 FANCD2  
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
8 RAD51  
Affinity Capture-Western Homo sapiens
9 HELQ  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 RAD18  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which POLN is involved
PathwayEvidenceSource
DNA Repair TAS Reactome
Fanconi Anemia Pathway TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here