Gene description for Chmp5
Gene name charged multivesicular body protein 5
Gene symbol Chmp5
Other names/aliases 2210412K09Rik
AW545668
Species Mus musculus
 Database cross references - Chmp5
ExoCarta ExoCarta_76959
Vesiclepedia VP_76959
Entrez Gene 76959
UniProt Q9D7S9  
 Chmp5 identified in sEVs derived from the following tissue/cell type
Embryonic fibroblasts 18494037    
 Gene ontology annotations for Chmp5
Biological Process
    plasma membrane repair GO:0001778 ISO
    regulation of receptor recycling GO:0001919 IMP
    vesicle budding from membrane GO:0006900 IBA
    autophagy GO:0006914 ISO
    nucleus organization GO:0006997 ISO
    lysosome organization GO:0007040 IMP
    mitotic metaphase chromosome alignment GO:0007080 ISO
    endosome to lysosome transport GO:0008333 IMP
    regulation of centrosome duplication GO:0010824 IEA
    regulation of centrosome duplication GO:0010824 ISO
    protein transport GO:0015031 IEA
    erythrocyte differentiation GO:0030218 IDA
    erythrocyte differentiation GO:0030218 IMP
    nuclear membrane reassembly GO:0031468 ISO
    late endosome to vacuole transport via multivesicular body sorting pathway GO:0032511 IBA
    multivesicular body assembly GO:0036258 NAS
    viral budding via host ESCRT complex GO:0039702 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    viral budding GO:0046755 ISO
    viral budding from plasma membrane GO:0046761 ISO
    vesicle fusion with vacuole GO:0051469 NAS
    multivesicular body-lysosome fusion GO:0061763 NAS
    multivesicular body-lysosome fusion GO:0061763 TAS
    midbody abscission GO:0061952 ISO
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to lipopolysaccharide GO:0071222 ISS
    cellular response to muramyl dipeptide GO:0071225 ISO
    cellular response to muramyl dipeptide GO:0071225 ISS
    multivesicular body sorting pathway GO:0071985 ISO
    membrane fission GO:0090148 NAS
    autophagosome maturation GO:0097352 ISO
    regulation of mitotic spindle assembly GO:1901673 ISO
    late endosome to lysosome transport GO:1902774 ISO
Subcellular Localization
    autophagosome membrane GO:0000421 ISO
    kinetochore GO:0000776 ISO
    nucleus GO:0005634 IDA
    nuclear pore GO:0005643 ISO
    lysosomal membrane GO:0005765 ISO
    multivesicular body GO:0005771 IBA
    kinetochore microtubule GO:0005828 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    midbody GO:0030496 ISO
    multivesicular body membrane GO:0032585 ISO
    amphisome membrane GO:1904930 ISO
 Experiment description of studies that identified Chmp5 in sEVs
1
Experiment ID 73
MISEV standards
EM
Biophysical techniques
HSP70|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
 Protein-protein interactions for Chmp5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vps4b 20479
Co-fractionation Mus musculus
2 Usp8  
Co-fractionation Mus musculus
3 2610002M06Rik  
Co-fractionation Mus musculus
4 Chmp1a  
Co-fractionation Mus musculus
5 Ndn  
Two-hybrid Mus musculus
6 Chmp2b  
Co-fractionation Mus musculus
7 Mitd1 69028
Co-fractionation Mus musculus
8 Chmp3  
Co-fractionation Mus musculus
9 Unk  
Affinity Capture-RNA Mus musculus
10 Tmem173  
Proximity Label-MS Mus musculus
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