Gene description for MKL1
Gene name megakaryoblastic leukemia (translocation) 1
Gene symbol MKL1
Other names/aliases BSAC
MAL
MRTF-A
Species Homo sapiens
 Database cross references - MKL1
ExoCarta ExoCarta_57591
Entrez Gene 57591
HGNC 14334
MIM 606078
UniProt Q969V6  
 MKL1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for MKL1
Molecular Function
    protein binding GO:0005515 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    leucine zipper domain binding GO:0043522 IPI
    actin monomer binding GO:0003785 ISS
    transcription coactivator activity GO:0003713 ISS
    actin binding GO:0003779 IDA
    transcription regulatory region sequence-specific DNA binding GO:0000976 IEA
Biological Process
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    positive regulation of transcription via serum response element binding GO:0010735 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    transcription, DNA-templated GO:0006351 IEA
    smooth muscle cell differentiation GO:0051145 IMP
    negative regulation of apoptotic signaling pathway GO:2001234 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified MKL1 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MKL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYOCD  
Reconstituted Complex Homo sapiens
2 SRF  
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
3 MKL2  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which MKL1 is involved
PathwayEvidenceSource
RHO GTPases Activate Formins TAS Reactome





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