Gene description for ANKRD1
Gene name ankyrin repeat domain 1 (cardiac muscle)
Gene symbol ANKRD1
Other names/aliases ALRP
C-193
CARP
CVARP
MCARP
bA320F15.2
Species Homo sapiens
 Database cross references - ANKRD1
ExoCarta ExoCarta_27063
Vesiclepedia VP_27063
Entrez Gene 27063
HGNC 15819
MIM 609599
UniProt Q15327  
 ANKRD1 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ANKRD1
Molecular Function
    p53 binding GO:0002039 IPI
    DNA binding GO:0003677 IDA
    transcription coactivator activity GO:0003713 IDA
    transcription corepressor activity GO:0003714 TAS
    protein binding GO:0005515 IPI
    titin binding GO:0031432 IPI
    histone deacetylase binding GO:0042826 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IBA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    R-SMAD binding GO:0070412 IPI
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    skeletal muscle cell differentiation GO:0035914 IEA
    response to muscle stretch GO:0035994 IMP
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of DNA damage response, signal transduction by p53 class mediator GO:0043517 IDA
    sarcomere organization GO:0045214 NAS
    negative regulation of DNA-templated transcription GO:0045892 IEA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of protein secretion GO:0050714 IMP
    cardiac muscle tissue morphogenesis GO:0055008 IMP
    cellular response to lipopolysaccharide GO:0071222 IDA
    cellular response to mechanical stimulus GO:0071260 IDA
    cellular response to interleukin-1 GO:0071347 IDA
    cellular response to tumor necrosis factor GO:0071356 IDA
    cellular response to xenobiotic stimulus GO:0071466 IEA
    cellular response to transforming growth factor beta stimulus GO:0071560 IDA
    phospholipase C/protein kinase C signal transduction GO:0141212 IEA
    negative regulation of DNA biosynthetic process GO:2000279 IMP
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    I band GO:0031674 ISS
 Experiment description of studies that identified ANKRD1 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ANKRD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MEOX2  
Two-hybrid Homo sapiens
2 MYPN 84665
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
3 TTN 7273
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 CDCA7L  
Two-hybrid Homo sapiens
5 SPANXN2  
Two-hybrid Homo sapiens
6 TRIM55  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
7 UBE2O 63893
Affinity Capture-MS Homo sapiens
8 TRIM63  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
9 NAGK 55577
Two-hybrid Homo sapiens
View the network image/svg+xml



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