Gene description for Csnk2a2
Gene name casein kinase 2, alpha prime polypeptide
Gene symbol Csnk2a2
Other names/aliases 1110035J23Rik
C77789
CK2
Species Mus musculus
 Database cross references - Csnk2a2
ExoCarta ExoCarta_13000
Vesiclepedia VP_13000
Entrez Gene 13000
UniProt O54833  
 Csnk2a2 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Csnk2a2
Molecular Function
    protein kinase activity GO:0004672 IEA
    kinase activity GO:0016301 IEA
    transferase activity GO:0016740 IEA
    nucleotide binding GO:0000166 IEA
    protein serine/threonine kinase activity GO:0004674 IDA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    protein N-terminus binding GO:0047485 ISO
Biological Process
    apoptotic process GO:0006915 IEA
    regulation of cell cycle GO:0051726 IDA
    phosphorylation GO:0016310 IEA
    mitotic spindle checkpoint GO:0071174 ISO
    regulation of mitochondrion degradation GO:1903146 ISO
    cell cycle GO:0007049 IEA
    Wnt signaling pathway GO:0016055 IEA
    transcription, DNA-templated GO:0006351 IEA
    regulation of transcription, DNA-templated GO:0006355 IEA
    protein phosphorylation GO:0006468 IDA
    positive regulation of protein targeting to mitochondrion GO:1903955 ISO
Subcellular Localization
    protein kinase CK2 complex GO:0005956 TAS
    PcG protein complex GO:0031519 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Csnk2a2 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 263
MISEV standards
✘ EV Biophysical techniques
✔
CD63|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Csnk2a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
2 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml
 Pathways in which Csnk2a2 is involved
PathwayEvidenceSource
Condensation of Prometaphase Chromosomes IEA Reactome
Signal transduction by L1 IEA Reactome
WNT mediated activation of DVL IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here