Gene description for Cul5
Gene name cullin 5
Gene symbol Cul5
Other names/aliases 4921514I20Rik
8430423K24Rik
AI852817
C030032G03Rik
C330021I08Rik
VACM-1
VACM1
Species Mus musculus
 Database cross references - Cul5
ExoCarta ExoCarta_75717
Vesiclepedia VP_75717
Entrez Gene 75717
UniProt Q9D5V5  
 Cul5 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cul5
Molecular Function
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein binding GO:0005515 IPI
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    cerebral cortex radially oriented cell migration GO:0021799 IMP
    radial glia guided migration of Purkinje cell GO:0021942 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    cytosolic calcium ion homeostasis GO:0051480 ISO
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    protein ubiquitination GO:0016567 IGI
Subcellular Localization
    cullin-RING ubiquitin ligase complex GO:0031461 IEA
    nucleus GO:0005634 ISO
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    Cul5-RING ubiquitin ligase complex GO:0031466 ISO
 Experiment description of studies that identified Cul5 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cul5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
2 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
View the network image/svg+xml
 Pathways in which Cul5 is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
Signaling by ERBB2 IEA Reactome





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