Gene description for Krt77
Gene name keratin 77
Gene symbol Krt77
Other names/aliases 4732484G22Rik
K1emb
Krt239
mK1b
Species Mus musculus
 Database cross references - Krt77
ExoCarta ExoCarta_406220
Vesiclepedia VP_406220
Entrez Gene 406220
UniProt Q6IFZ6  
 Krt77 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
Oligodendrocytes 21136642    
 Gene ontology annotations for Krt77
Molecular Function
    structural constituent of skin epidermis GO:0030280 IBA
Biological Process
    keratinization GO:0031424 IBA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cornified envelope GO:0001533 IDA
    cytoskeleton GO:0005856 ISO
    keratin filament GO:0045095 IBA
    keratin filament GO:0045095 IEA
 Experiment description of studies that identified Krt77 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for Krt77
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nphp3  
Affinity Capture-MS Mus musculus
2 Dsc1  
Co-fractionation Mus musculus
3 Uba5  
Co-fractionation Mus musculus
4 Myh4  
Co-fractionation Mus musculus
5 Nphp4  
Affinity Capture-MS Mus musculus
6 Mks1  
Affinity Capture-MS Mus musculus
7 Ankfy1 11736
Co-fractionation Mus musculus
8 Cbr1  
Co-fractionation Mus musculus
9 Ahi1  
Affinity Capture-MS Mus musculus
10 Nphp1  
Affinity Capture-MS Mus musculus
11 Dmrt2  
Affinity Capture-MS Mus musculus
12 Iqcb1  
Affinity Capture-MS Mus musculus
13 Cul5 75717
Co-fractionation Mus musculus
14 Arg1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Krt77 is involved
PathwayEvidenceSource
Developmental Biology IEA Reactome
Formation of the cornified envelope IEA Reactome
Keratinization IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here